PD-L1 蛋白, Canine (HEK293, Fc)

PD-L1 Protein, Canine (HEK293, Fc) is a trans-membrane protein that is considered to be a co-inhibitory factor of the immune response. The amino acid sequence of PD-L1 is encoded by 7 exons, which form a protein of ~40 kDa. PD-L1 is a type I transmembrane protein, is part of the immunoglobulin (Ig) superfamily and is composed of IgV-like and IgC-like extracellular domains, a hydrophobic transmembrane domain and a short cytoplasmic tail composed of 30 amino acids. PD-L1 can combine with PD-1 to reduce the proliferation of PD-1 positive cells, inhibit their cytokine secretion and induce apoptosis. PD-L1 also plays an important role in various malignancies where it can attenuate the host immune response to tumor cells^[1][2]. PD-L1 Protein, Canine (HEK293, Fc) is the recombinant canine-derived PD-L1 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of PD-L1 Protein, Canine (HEK293, Fc) is 218 a.a., with molecular weight of ~70-80 & 140-160 kDa, respectively.

PD-L1 是 B7 家族的第三个成员，不结合 CD28、细胞毒性 T 淋巴细胞 A4 或诱导型共刺激剂，与 B7.1 和 B7.2 蛋白具有 10-25% 的同源性。PD-L1 由 PDCDL1 基因编码，该基因被发现位于人类 9 号染色体的 p24.1 处^[1]。
PD-L1 最重要的作用是与程序性死亡-1 (PD-1；CD279) 结合，程序性死亡-1 是一种 I 型跨膜受体，长度为 288 个氨基酸，首次在 T 细胞上发现。PD-L1 和 PD-1 与癌细胞的结合会激活包含 Src 同源区 2 结构域的磷酸酶，从而抑制 T 细胞受体 (TCR) 通路。TCR 通路的抑制会导致 T 细胞活性的抑制，包括增殖、存活和细胞因子的产生，例如 IL-2、肿瘤坏死因子 α (TNF-α) 和干扰素 γ (IFN-γ) 以及 B7-1 和 T 细胞耐受性的抑制^[1]。

Measured by its binding ability in a functional ELISA. When Recombinant Canine PD-1 is present at 0.5 μg/mL, can bind Recombinant Canine PD-L1. The ED₅₀ for this effect is 4.116 μg/mL.

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  Measured by its binding ability in a functional ELISA. When Recombinant Canine PD-1 is present at 0.5 μg/mL, can bind Recombinant Canine PD-L1. The ED₅₀ for this effect is 4.116 μg/mL.

Canine

HEK293

C-hFc

NP_001278901 (F19-R236)

484186  [NCBI]

FTITVSKDLYVVEYGGNVTMECKFPVEKQLNLFALIVYWEMEDKKIIQFVNGKEDLKVQHSSYSQRAQLLKDQLFLGKAALQITDVRLQDAGVYCCLIGYGGADYKRITLKVHAPYRNISQRISVDPVTSEHELMCQAEGYPEAEVIWTSSDHRVLSGKTTITNSNREEKLFNVTSTLNINATANEIFYCTFQRSGPEENNTAELVIPERLPVPASER

Approximately 70-80&140-160 kDa due to the glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.