PD-L1 蛋白, Canine (HEK293, His)

The PD-L1 protein critically regulates immune tolerance by acting as a ligand for PDCD1/PD-1, regulating T cell activation threshold, and limiting effector responses. It may act as a costimulatory molecule for IL10-producing T cell subsets. PD-L1 Protein, Canine (HEK293, His) is the recombinant canine-derived PD-L1 protein, expressed by HEK293 , with C-His labeled tag. The total length of PD-L1 Protein, Canine (HEK293, His) is 218 a.a., with molecular weight (glycosylation form) of 50 & 100 kDa, respectively.

PD-L1 蛋白在诱导和维持自身免疫耐受方面发挥着关键作用，作为抑制性受体 PDCD1/PD-1 的配体，从而调节 T 细胞的激活阈值，最终限制其效应反应。此外，PD-L1 可能作为 T 细胞亚群的共刺激分子，主要通过尚未鉴定的激活受体产生白细胞介素 10 (IL10)。除了作为免疫检查点的作用外，PD-L1 还充当转录共激活因子，在缺氧时易位到细胞核中，并与磷酸化的 STAT3 相互作用，促进 GSDMC 的转录，导致细胞焦亡。PD-L1 与 PDCD1/PD-1 相互作用促进的 PDCD1 介导的抑制途径可抑制细胞毒性 T 淋巴细胞 (CTL) 效应功能，被肿瘤利用来减弱抗肿瘤免疫力并逃避免疫系统破坏。阻断 PDCD1 介导的途径已显示出逆转耗尽的 T 细胞表型和使抗肿瘤反应正常化的希望，为癌症免疫治疗提供了理论依据。

Canine

HEK293

C-His

E2RKZ5 (F19-R236)

484186  [NCBI]

50 &100 kDa. The reducing (R) protein migrates as 50&100 kDa in SDS-PAGE may be due to glycosylation and multimer structure.

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized a 0.22 μm filtered solution of 20 mM Tris-HC1, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.