PD-L1 蛋白, Cynomolgus (HEK293, His)

CD274 molecule, also known as programmed death ligand 1 (PD-L1), binds to the checkpoint suppressor molecule PD-1 to inhibit TCR-mediated IL-2 production and T cell proliferation signaling. PD-L1 is involved in the PI3K/JAK/STAT signaling pathway to promote tumor occurrence. PD-L1 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived PD-L1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of PD-L1 Protein, Cynomolgus (HEK293, His) is 221 a.a., with molecular weight of 32-40 kDa.

CD274 molecule 也被称为程序性死亡配体 1 (PD-L1)，PD-L1与抑制检查点分子 PD-1 结合，通过免疫受体酪氨酸基开关 Motif (ITSM) 与磷酸酶 (SHP-1或SHP-2) 相互作用，传递抑制信号。PD-L1 与其受 PD-1 在 T 细胞上的结合可传递抑制 TCR 介导的 IL-2 产生和 T 细胞增殖的信号。通过抑制 ZAP70 磷酸化及其与 CD3ζ 的关联。PD-1 信号减弱 PKC-θ 激活环磷酸化 (由 TCR 信号引起)，这是激活转录因子 NF-κB 和 AP-1 以及产生 IL-2 所必需的。PD-L1 与 PD-1 的结合也通过诱导 E3 泛素连接酶 CBL-b 的上调。PD-L1 参与 PI3K/JAK/STAT 信号通路促进肿瘤的发生。

Cynomolgus

HEK293

C-6*His

G7PSE7 (F19-T239)

102145573  [NCBI]

FTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLTSLIVYWEMEDKNIIQFVHGEEDLKVQHSNYRQRAQLLKDQLSLGNAALRITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLLNVTSTLRINTTANEIFYCIFRRLDPEENHTAELVIPELPLALPPNERT

32-40 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.