PDCD4 蛋白, Human (His)

PDCD4 inhibits translation initiation by disrupting the EIF4A1-EIF4G interaction and inhibiting EIF4A helicase activity. It regulates JUN kinase activation and downregulates MAP4K1, inhibiting invasion and tumorigenesis. PDCD4 Protein, Human (His) is the recombinant human-derived PDCD4 protein, expressed by E. coli , with C-6*His labeled tag.

PDCD4 作为一种多面蛋白，可能通过破坏 EIF4A1 和 EIF4G 之间的相互作用并阻碍 EIF4A 的解旋酶活性，在抑制翻译起始和帽子依赖性翻译中发挥关键作用。此外，它还调节 JUN 激酶激活并下调 MAP4K1 表达，从而抑制与侵袭和肿瘤发生相关的关键事件。作为肿瘤抑制因子，PDCD4 具有阻碍肿瘤启动子诱导的肿瘤转化的能力。它结合 RNA 并与 EIF4A1、EIF4A2 和 EIF4G1 相互作用，形成影响翻译机制的复合物。PDCD4 的磷酸化促进了与 BTRC 和 FBXW11 的相互作用，为其在细胞过程（包括细胞凋亡和肿瘤抑制）中的调节功能增加了一层复杂性。

Human

E. coli

C-6*His

Q53EL6 (K212-P357)

27250  [NCBI]

KASHREMTSKLLSDLCGTVMSTTDVEKSFDKLLKDLPELALDTPRAPQLVGQFIARAVGDGILCNTYIDSYKGTVDCVQARAALDKATVLLSMSKGGKRKDSVWGSGGGQQSVNHLVKEIDMLLKEYLLSGDISEAEHCLKELEVP

Approximately 17.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.