PDCD8/AIFM1 蛋白, Human (His)

PDCD8/AIFM1 protein has the dual functions of NADH oxidoreductase and apoptosis regulator, and translocates from the mitochondrial intermembrane space to the cytoplasm and nucleus in response to apoptotic stimuli. It acts through a caspase-independent pathway, inducing “parthanatos” characterized by fragmentation of chromosomal DNA. PDCD8/AIFM1 Protein, Human (His) is the recombinant human-derived PDCD8/AIFM1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of PDCD8/AIFM1 Protein, Human (His) is 493 a.a., with molecular weight of ~68.0 kDa.

PDCD8/AIFM1 蛋白表现出双重功能，既充当 NADH 氧化还原酶又充当细胞凋亡调节剂。为了响应凋亡刺激，它从线粒体膜间隙易位到胞质和细胞核，通过不依赖半胱天冬酶的途径发挥促凋亡因子的作用。其与聚 ADP 核糖链的结合促进了这种释放到细胞质中。细胞核中发现的可溶形式 (AIFsol) 会诱导“parthanatos”，其特征是染色体 DNA 发生不依赖半胱天冬酶的断裂。此外，PDCD8/AIFM1 与 EIF3G 相互作用，抑制 EIF3 机制和蛋白质合成，同时激活 caspase-7 以放大细胞凋亡。它在过氧化氢诱导的不依赖半胱天冬酶的固缩细胞死亡中发挥着关键作用。在正常线粒体代谢中，PDCD8/AIFM1 通过与 CHCHD4 相互作用并控制 CHCHD4 线粒体输入，对呼吸链生物发生的调节做出显着贡献。值得注意的是，PDCD8/AIFM1 表现出 NADH 氧化还原酶活性，并且不诱导核细胞凋亡。

Human

E. coli

N-6*His

O95831 (E121-D613)

9131  [NCBI]

EEVPQDKAPSHVPFLLIGGGTAAFAAARSIRARDPGARVLIVSEDPELPYMRPPLSKELWFSDDPNVTKTLRFKQWNGKERSIYFQPPSFYVSAQDLPHIENGGVAVLTGKKVVQLDVRDNMVKLNDGSQITYEKCLIATGGTPRSLSAIDRAGAEVKSRTTLFRKIGDFRSLEKISREVKSITIIGGGFLGSELACALGRKARALGTEVIQLFPEKGNMGKILPEYLSNWTMEKVRREGVKVMPNAIVQSVGVSSGKLLIKLKDGRKVETDHIVAAVGLEPNVELAKTGGLEIDSDFGGFRVNAELQARSNIWVAGDAACFYDIKLGRRRVEHHDHAVVSGRLAGENMTGAAKPYWHQSMFWSDLGPDVGYEAIGLVDSSLPTVGVFAKATAQDNPKSATEQSGTGIRSESETESEASEITIPPSTPAVPQAPVQGEDYGKGVIFYLRDKVVVGIVLWNIFNRMPIARKIIKDGEQHEDLNEVAKLFNIHED

Approximately 68.0 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.