1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. PDE4D 蛋白, Human (His)

PDE4D 蛋白, Human (His)

目录号: HY-P701961
COA 产品使用指南

PDE4D(磷酸二酯酶 4D)蛋白作为一种水解酶,可降解第二信使 cAMP,而 cAMP 是各种生理过程中的关键调节剂。其分解 cAMP 的酶活性强调了 PDE4D 在调节细胞信号级联、维持由这一关键第二信使介导的细胞内通讯的复杂平衡方面的关键作用。PDE4D 蛋白, Human (His) 是重组的 PDE4D 蛋白,由 E. coli 表达,带有 N-6*His 标签。PDE4D 蛋白, Human (His) 全长 328 个氨基酸。

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规格 价格 是否有货 数量
10 μg ¥2050 In-stock
50 μg ¥5800 询价
100 μg   询价  

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描述

PDE4D (Phosphodiesterase 4D) protein, as a hydrolytic enzyme, degrades the second messenger cAMP, a crucial regulator in various physiological processes. Its enzymatic activity in breaking down cAMP underscores PDE4D's pivotal role in modulating cellular signaling cascades, maintaining the intricate balance of intracellular communication mediated by this critical second messenger. PDE4D Protein, Human (His) is the recombinant human-derived PDE4D protein, expressed by E. coli , with N-6*His labeled tag. The total length of PDE4D Protein, Human (His) is 328 a.a., .

研究背景

PDE4D(磷酸二酯酶 4D)蛋白充当水解酶,负责第二信使 cAMP 的降解。这种分子,环磷酸腺苷 (cAMP),在许多重要的生理过程中起着至关重要的调节作用。PDE4D 分解 cAMP 的酶活性强调了其在调节细胞信号级联和维持由这一关键第二信使介导的细胞内通讯的复杂平衡中的关键作用。

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

Q08499 (T388-S715)

基因 ID

5144

蛋白结构
N-term
6*His
PDE4D (T388-S715)
Accession # Q08499
C-term
同用名
PDE4D; cAMP-specific 3'; 5'-cyclic phosphodiesterase 4D; DPDE3; PDE43
分子量

Approximately 40 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

溶液

组分

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH 7.5, 200 mM NaCl, 20% glycerol, 1mM DTT.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

Please use rapid thawing with running water to thaw the protein.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

PDE4D Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
PDE4D Protein, Human (His)
目录号:
HY-P701961
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