PDGF-BB 蛋白, Human (His)

The PDGF-BB protein is a key growth factor that centrally regulates embryonic development, cell proliferation, migration, survival, and chemotaxis. It is known for its potent mitogenic effects on mesenchymal cells and is essential for the normal proliferation and recruitment of pericytes and vascular smooth muscle cells in tissues such as the central nervous system, skin, lungs, heart, and placenta. PDGF-BB Protein, Human (His) is the recombinant human-derived PDGF-BB protein, expressed by E. coli , with N-6*His labeled tag. The total length of PDGF-BB Protein, Human (His) is 109 a.a., with molecular weight of approximately 15 kDa.

GMP PDGF-BB 蛋白是一种关键的生长因子，在调节胚胎发育、细胞增殖、迁移、存活和趋化性方面发挥着核心作用。GMP PDGF-BB 以其对间充质细胞的强有丝分裂作用而闻名，对于各种组织（包括中枢神经系统、皮肤、肺、心脏和胎盘）中周细胞和血管平滑肌细胞的正常增殖和募集是不可或缺的。它的重要贡献延伸到血管和肾小球的发育，突出了它在血管和肾脏生理学中的重要性。GMP PDGF-BB 是伤口愈合的关键参与者，其信号动力学通过与 PDGFA 形成异二聚体进行微调。GMP PDGF-BB 作为反平行同二聚体存在，与 PDGFRA 和 PDGFRB 同二聚体以及由 PDGFRA 和 PDGFRB 形成的异二聚体进行二硫键连接的相互作用。此外，它还与 PDGFA 形成反向平行异二聚体，进一步使其调控手段多样化。值得注意的是，GMP PDGF-BB 与 XLKD1、LRP1 和 SORL1 建立连接，有助于形成调节其多方面功能的相互作用网络。

Measured in a cell proliferation assay using NIH3T3 mouse fibroblast cells. The ED₅₀ for this effect is ≤5.712 ng/mL, corresponding to a specific activity is ≥1.751×10⁵ units/mg.

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  Measured in a cell proliferation assay using NIH3T3 mouse fibroblast cells. The ED₅₀ for this effect is 5.712 ng/mL, corresponding to a specific activity is 1.751×10⁵ units/mg.

Human

E. coli

N-6*His

P01127-1 (S82-T190)

5155  [NCBI]

SLGSLTIAEPAMIAECKTRTEVFEISRRLIDRTNANFLVWPPCVEVQRCSGCCNNRNVQCRPTQVQLRPVQVRKIEIVRKKPIFKKATVTLEDHLACKCETVAAARPVT

approximately 15 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, 200 mM arginine, pH 8.0 or 50 mM Tris-HCL, 300 mM NaCl, 200 mM arginine, pH 8.0, 5% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.