PDGF R beta 蛋白, Human (Biotinylated, HEK293, His-Avi)

PDGF Rβ protein is integral in embryonic development and regulates cell proliferation, survival, differentiation, chemotaxis, migration, and vascular development. It crucially recruits pericytes and smooth muscle cells to the endothelium, promoting vascular smooth muscle cell migration and neointimal formation at the site of injury. PDGF R beta Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived PDGF R beta protein, expressed by HEK293 , with C-Avi, C-His labeled tag.

文中描述的受体是血小板源性生长因子受体α（PDGFRA）。PDGFRA参与多种细胞过程，包括胚胎发育、细胞增殖、存活、分化、趋化性、迁移和血管发育。它在周细胞和平滑肌细胞向内皮细胞的募集、血管平滑肌细胞的迁移和血管损伤部位新内膜的形成中发挥着至关重要的作用。PDGFRA 是心血管系统正常发育和肾小球毛细血管分支网络形成所必需的。它促进肌动蛋白细胞骨架的重排和膜褶皱的形成。其配体（包括同型二聚体 PDGFB、PDGFA 和 PDGFB 形成的异二聚体或同型二聚体 PDGFD）的结合会导致多个信号级联的激活。PDGFRA 磷酸化各种下游效应子，包括 PLCG1、PIK3R1、PTPN11、RASA1/GAP、CBL、SHC1、NCK1、PDCD6IP/ALIX 和 STAM。受体信号传导受到使受体及其下游效应子去磷酸化的蛋白磷酸酶以及激活受体的快速内化的调节。

1.The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
2.Immobilized Anti-PDGF R beta Antibody, hFc Tag at 2 μg/mL (100 μl/well) on the plate. Dose response curve for Biotinylated Human PDGF R beta, His Tag with the EC₅₀ of ≤5 ng/mL determined by ELISA.

Human

HEK293

C-Avi;C-His

P09619-1 (L33-F530)

5159  [NCBI]

78-115 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.