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  3. PDILT 蛋白, Human (HEK293, His)

PDILT 蛋白, Human (HEK293, His)

目录号: HY-P71194
产品使用指南

PDILT 蛋白可能是精子发生过程中必需的氧化还原失活伴侣,形成非二硫键连接的同二聚体。它的作用强调了它在男性生殖细胞发育中的重要性。PDILT 蛋白, Human (HEK293, His) 是重组的 PDILT 蛋白,由 HEK293 表达,带有 C-6*His 标签。PDILT 蛋白, Human (HEK293, His) 全长 564 个氨基酸,分子量约为 ~92.0 kDa。

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描述

The PDILT protein is a possible redox-inactive chaperone essential in spermatogenesis, forming non-disulfide-linked homodimers. Its role emphasizes its importance in male germ cell development. PDILT Protein, Human (HEK293, His) is the recombinant human-derived PDILT protein, expressed by HEK293 , with C-6*His labeled tag. The total length of PDILT Protein, Human (HEK293, His) is 564 a.a., with molecular weight of ~92.0 kDa.

研究背景

PDILT 蛋白被鉴定为可能参与精子发生的氧化还原无活性伴侣。该伴侣以同二聚体形式存在,在二聚体结构内不形成二硫键。它在精子发生中的作用强调了它在与男性生殖细胞发育相关的复杂过程中的重要性。此外,PDILT 与 ERO1A 和 CLGN 相互作用,表明与参与氧化还原调节和伴侣活性的蛋白质存在潜在关联。深入探讨 PDILT 在精子发生中的具体分子机制和功能意义及其与 ERO1A 和 CLGN 的相互作用,将有助于全面了解其在男性生殖生物学中的作用。

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

Q8N807 (S21-L584)

基因 ID

204474  [NCBI]

蛋白结构
N-term
PDILT (S21-L584)
Accession # Q8N807
6*His
C-term
同用名
Protein Disulfide-Isomerase-Like Protein of the Testis; PDILT
氨基酸序列

SPEVNAGVSSIHITKPVHILEERSLLVLTPAGLTQMLNQTRFLMVLFHNPSSKQSRNLAEELGKAVEIMGKGKNGIGFGKVDITIEKELQQEFGITKAPELKLFFEGNRSEPISCKGVVESAALVVWLRRQISQKAFLFNSSEQVAEFVISRPLVIVGFFQDLEEEVAELFYDVIKDFPELTFGVITIGNVIGRFHVTLDSVLVFKKGKIVNRQKLINDSTNKQELNRVIKQHLTDFVIEYNTENKDLISELHIMSHMLLFVSKSSESYGIIIQHYKLASKEFQNKILFILVDADEPRNGRVFKYFRVTEVDIPSVQILNLSSDARYKMPSDDITYESLKKFGRSFLSKNATKHQSSEEIPKYWDQGLVKQLVGKNFNVVVFDKEKDVFVMFYAPWSKKCKMLFPLLEELGRKYQNHSTIIIAKIDVTANDIQLMYLDRYPFFRLFPSGSQQAVLYKGEHTLKGFSDFLESHIKTKIEDEDELLSVEQNEVIEEEVLAEEKEVPMMRKGLPEQQSPELENMTKYVSKLEEPAGKKKTSEEVVVVVAKPKGPPVQKKKPKVKEEL

分子量

Approximately 92.0 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

PDILT Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
PDILT Protein, Human (HEK293, His)
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HY-P71194
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