1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. PDK3 蛋白, Mouse (His)

PDK3 蛋白, Mouse (His)

目录号: HY-P71614
产品使用指南

PDK3 是一种代谢调节剂,通过 PDHA1 磷酸化抑制丙酮酸脱氢酶,从而微调葡萄糖代谢并影响有氧呼吸。PDHA2 的磷酸化增加了另一层控制。PDK3 蛋白, Mouse (His) 是重组的 PDK3 蛋白,由 E. coli 表达,带有 N-His 标签。

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描述

PDK3 is a metabolic regulator that inhibits pyruvate dehydrogenase through PDHA1 phosphorylation to fine-tune glucose metabolism and affect aerobic respiration. Phosphorylation of PDHA2 adds another layer of control. PDK3 Protein, Mouse (His) is the recombinant mouse-derived PDK3 protein, expressed by E. coli , with N-His labeled tag.

研究背景

PDK3 是代谢调节的关键参与者,通过 E1 亚基 PDHA1 的磷酸化抑制丙酮酸脱氢酶活性来发挥其影响,从而微调葡萄糖代谢和有氧呼吸。它磷酸化 PDHA2 的能力为这些关键的细胞过程增加了另一层控制。在长时间禁食或高脂肪饮食的情况下,PDK3 会协调代谢转变,减少葡萄糖利用率并增强脂肪代谢。除了在营养适应方面的作用外,PDK3 还成为葡萄糖稳态的关键贡献者,在维持正常血糖水平方面发挥着至关重要的作用。此外,它参与活性氧的产生,为其多方面的功能增添了一层复杂性。

种属

Mouse

表达系统

E. coli

标签

N-His

蛋白编号

Q922H2 (I131-S362)

基因 ID
蛋白结构
N-term
His
PDK3 (I131-S362)
Accession # Q922H2
C-term
同用名
Pdk3; Pyruvate dehydrogenase kinase isoform 3
氨基酸序列

IEYKEKFGFDPFISSNIQYFLDRFYTNRISFRMLINQHTLLFGGDTNPAHPKHIGSIDPTCNVADVVKDAYETAKMLCEQYYLVAPELEVEEFNAKAPNKPIQVVYVPSHLFHMLFELFKNSMRATVELHEDKKEGYPAVKTLVTLGKEDLSIKISDLGGGVPLRKIDRLFNYMYSTAPRPSLEPTRAAPLAGFGYGLPISRLYARYFQGDLKLYSMEGVGTDAVIYLKALS

分子量

Approximately 30.3 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

PDK3 Protein, Mouse (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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