PEDF 蛋白, Human

PEDF Protein, Human is a secreted glycoprotein and a non-inhibitory member of the serine protease inhibitor (serpin) family.

色素上皮衍生因子属于丝氨酸蛋白酶抑制剂 (serpin) 家族。色素上皮衍生因子基因包含典型的信号肽序列、起始甲硫氨酸密码子和聚腺苷酸化信号，并且与丝氨酸蛋白酶抑制剂超家族其他成员的大小相匹配。色素上皮衍生因子是一种神经营养剂^[1]。它在人类胎儿和成人组织中广泛表达，但其表达随着年龄的增长和恶性组织而降低。色素上皮衍生因子的主要抗癌活性源自其双重作用，即间接作用于肿瘤微环境（间接抗肿瘤作用）或直接作用于肿瘤本身（直接抗肿瘤作用）^[2]。色素上皮衍生因子具有复杂的神经营养、神经保护、抗血管生成、抗氧化和抗炎特性，有可能被用作治疗糖尿病血管并发症的治疗选择^[3]。

The ED₅₀ is <2 ng/mL as measured by human Saos2 cells, corresponding to a specific activity of >5.0 × 10⁵ IU/mg.

Human

E. coli

Tag Free

P36955 (Q20-P418)

5176  [NCBI]

QNPASPPEEGSPDPDSTGALVEEEDPFFKVPVNKLAAAVSNFGYDLYRVRSSTSPTTNVLLSPLSVATALSALSLGAEQRTESIIHRALYYDLISSPDIHGTYKELLDTVTAPQKNLKSASRIVFEKKLRIKSSFVAPLEKSYGTRPRVLTGNPRLDLQEINNWVQAQMKGKLARSTKEIPDEISILLLGVAHFKGQWVTKFDSRKTSLEDFYLDEERTVRVPMMSDPKAVLRYGLDSDLSCKIAQLPLTGSMSIIFFLPLKVTQNLTLIEESLTSEFIHDIDRELKTVQAVLTVPKLKLSYEGEVTKSLQEMKLQSLFDSPDFSKITGKPIKLTQVEHRAGFEWNEDGAGTTPSPGLQPAHLTFPLDYHLNQPFIFVLRDTDTGALLFIGKILDPRGP

40-49 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against 20 mM PB, pH 7.4, 150 mM NaCl.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Steele FR, et al. Pigment epithelium-derived factor: neurotrophic activity and identification as a member of the serine protease inhibitor gene family. Proc Natl Acad Sci U S A. 1993 Feb 15;90(4):1526-30.  [Content Brief]

  [2]. Belkacemi L, et al. Anti-tumor effects of pigment epithelium-derived factor (PEDF): implication for cancer therapy. A mini-review. J Exp Clin Cancer Res. 2016 Jan 8;35:4.  [Content Brief]

  [3]. Yamagishi S, et al. Pigment epithelium-derived factor (PEDF): its potential therapeutic implication in diabetic vascular complications. Curr Drug Targets. 2008 Nov;9(11):1025-9.  [Content Brief]