Peptidyl-prolyl cis-trans isomerase A/CYPA 蛋白, Human (Tag Free)

CYPA protein, a peptidyl prolyl cis-trans isomerase, has proinflammatory activity by stimulating activation of NF-kappa-B and ERK, JNK, and p38 MAP kinases. It may act as a mediator between the human SARS coronavirus nucleoprotein and BSG/CD147 during the virus' invasion of host cells. Peptidyl-prolyl cis-trans isomerase A/CYPA Protein, Human is the recombinant human-derived Peptidyl-prolyl cis-trans isomerase A/CYPA protein, expressed by E. coli , with tag free.

肽基脯氨酰顺反异构酶 A (CYPA) 催化寡肽中脯氨酸酰亚胺肽键的顺反异构化。该酶发挥多方面的作用，通过激活其膜受体 BSG/CD147 对白细胞产生强烈的趋化作用，启动信号级联反应，最终导致 MAPK/ERK 激活。此外，CYP A 以促炎方式激活内皮细胞 (EC)，诱导 NF-κ-B 和 MAP 激酶信号传导，并上调粘附分子。它诱导 EC 细胞凋亡，促进 CCL2 和 BCL2L11 的 FOXO1 依赖性表达。为了应对氧化应激，CYP A 在 EC 中启动促凋亡和抗凋亡信号传导。它负向调节 MAP3K5/ASK1 激酶活性，并且是异质核核糖核蛋白复合物中 TARDBP 组装所必需的，从而调节 TARDBP 与 RNA 的结合。CYP A 在血小板活化和聚集、调节钙动员以及促进整合素 ITGA2B:ITGB3 双向信号传导中也发挥着至关重要的作用。此外，它还能抑制甲型流感病毒的复制，并可能在病毒入侵宿主细胞期间充当 SARS 冠状病毒核蛋白和 BSG/CD147 之间的介质。

Measured by its ability to inhibit calcineurin phosphatase activity in the presence of Cyclosporin A. The IC₅₀ for inhibition of calcineurin activity is ≤420 nM that incubate at 37 ºC for 60 minutes.

Human

E. coli

Tag Free

P62937 (M1-E165)

5478

MVNPTVFFDIAVDGEPLGRVSFELFADKVPKTAENFRALSTGEKGFGYKGSCFHRIIPGFMCQGGDFTRHNGTGGKSIYGEKFEDENFILKHTGPGILSMANAGPNTNGSQFFICTAKTEWLDGKHVVFGKVKEGMNIVEAMERFGSRNGKTSKKITIADCGQLE

Approximately 16-18 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.