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  3. PFDN4 蛋白, Human (His)

PFDN4 蛋白, Human (His)

目录号: HY-P71198
产品使用指南

PFDN4 蛋白选择性地结合细胞质伴侣蛋白 (c-CPN) 并指导该蛋白进行靶向转移。它还与新生肽相互作用,并积极促进复杂细胞环境中的正确折叠。PFDN4 蛋白, Human (His) 是重组的 PFDN4 蛋白,由 E. coli 表达,带有 N-6*His 标签。PFDN4 蛋白, Human (His) 全长 134 个氨基酸,分子量为 18-20 kDa。

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描述

The PFDN4 protein selectively binds to the cytoplasmic chaperone protein (c-CPN) and directs the protein for targeted transfer. It also interacts with nascent peptides and actively promotes correct folding in complex cellular environments. PFDN4 Protein, Human (His) is the recombinant human-derived PFDN4 protein, expressed by E. coli , with N-6*His labeled tag. The total length of PFDN4 Protein, Human (His) is 134 a.a., with molecular weight of 18-20 kDa.

研究背景

PFDN4 蛋白与胞质伴侣蛋白 (c-CPN) 精确结合,促进蛋白质靶向转移至该伴侣。此外,PFDN4 与新生多肽链结合,积极促进其在以众多非天然蛋白质竞争途径为特征的细胞环境中正确折叠。PFDN4作为由两个PFD-α型和四个PFD-β型亚基组成的异六聚体,在细胞蛋白质稳态中发挥着至关重要的作用。值得注意的是,它以磷酸化依赖性方式与 URI1 相互作用,并且这种相互作用以生长依赖性方式调节,强调其细胞功能的动态性质。

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

Q9NQP4 (M1-S134)

基因 ID

5203  [NCBI]

蛋白结构
N-term
6*His
PFDN4 (M1-S134)
Accession # Q9NQP4
C-term
同用名
Prefoldin Subunit 4; Protein C-1; PFDN4; PFD4
氨基酸序列

MAATMKKAAAEDVNVTFEDQQKINKFARNTSRITELKEEIEVKKKQLQNLEDACDDIMLADDDCLMIPYQIGDVFISHSQEETQEMLEEAKKNLQEEIDALESRVESIQRVLADLKVQLYAKFGSNINLEADES

分子量

18-20 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

PFDN4 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
PFDN4 Protein, Human (His)
目录号:
HY-P71198
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