PFKM 蛋白, Human (HEK293, His)

PFKM protein, a key regulator in cellular metabolism, catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate, initiating glycolysis. This fundamental enzymatic step controls glucose entry into glycolysis, influencing downstream energy production and cellular metabolism. PFKM's pivotal role highlights its significance in orchestrating metabolic flux and maintaining cellular energy balance. PFKM Protein, Human (HEK293, His) is the recombinant human-derived PFKM protein, expressed by HEK293 , with C-6*His labeled tag.

PFKM 蛋白以 ATP 为底物，催化 D-果糖 6-磷酸磷酸化为果糖 1,6-二磷酸，从而在细胞代谢中发挥关键作用。这种酶活性标志着糖酵解的初始和关键步骤，糖酵解是能量产生的核心基本代谢途径。通过促进果糖 6-磷酸转化为果糖 1,6-二磷酸，PFKM 在调节葡萄糖进入糖酵解途径中发挥着关键作用，从而影响下游的能量产生和细胞代谢过程。PFKM 在催化这一重要反应中的重要性强调了其在协调代谢流和维持细胞能量平衡方面的重要性。

Measured by its ability to phosphorylate fructose-6-phosphate.The specific activity is 502.29 pmol/min/μg, as measured under the described conditions.

Human

HEK293

C-6*His

P08237-1 (T2-V780)

5213  [NCBI]

THEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Approximately 93.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 5 mM EDTA, 5% Trehalose, pH 6.9.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.