2. 重组蛋白
  3. Enzymes & Regulators
  4. Isomerases (EC 5)
  5. PGAM1 蛋白, Human

PGAM1 蛋白, Human

目录号: HY-P701962
COA 产品使用指南

PGAM1 蛋白通过催化 2-磷酸甘油酸和 3-磷酸甘油酸的重要相互转化，在糖酵解中发挥关键作用。此外，它有助于将 (2R)-2,3-二磷酸甘油酸转化为 (2R)-3-磷酸甘油酰磷酸，进一步促进与糖酵解途径相关的复杂代谢过程。PGAM1 蛋白, Human 是重组的 PGAM1 蛋白，由 E. coli 表达，不带标签。PGAM1 蛋白, Human 全长 254 个氨基酸。

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规格 价格 是否有货 数量
10 μg ￥2200 In-stock
50 μg ￥6200 询价
100 μg   询价  

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描述

PGAM1 protein plays a pivotal role in glycolysis by catalyzing the vital interconversion of 2-phosphoglycerate and 3-phosphoglycerate. Additionally, it is instrumental in the conversion of (2R)-2,3-bisphosphoglycerate to (2R)-3-phospho-glyceroyl phosphate, further contributing to the intricate metabolic processes associated with glycolytic pathways. PGAM1 Protein, Human is the recombinant human-derived PGAM1 protein, expressed by E. coli , with tag free. The total length of PGAM1 Protein, Human is 254 a.a., .
研究背景

PGAM1（或磷酸甘油酸变位酶 1）通过催化 2-磷酸甘油酸和 3-磷酸甘油酸的可逆相互转化，在糖酵解中发挥关键作用。这种酶活性是糖酵解途径中的关键步骤，促进葡萄糖衍生的底物转化为能量和代谢中间体。此外，PGAM1 还参与 (2R)-2,3-二磷酸甘油酸酯和 (2R)-3-磷酸甘油酰磷酸的相互转化。这些反应的调节对于维持糖酵解通量和确保代谢物通过糖酵解途径的适当流动至关重要。PGAM1 的酶功能有助于细胞内能量产生和代谢稳态的整体效率。
种属

Human
表达系统

E. coli
标签

Tag Free
蛋白编号

P18669 (M1-K254)
基因 ID

5223
蛋白结构
N-term
PGAM1 (M1-K254)
Accession # P18669
C-term
同用名
PGAM1; Phosphoglycerate mutase 1; BPG-dependent PGAM 1; Phosphoglycerate mutase isozyme B; PGAM-B
分子量

28.9 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
性状

溶液.
组分

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH7.5, 200 mM NaCl, 20% glycerol.
内毒素含量

<1 EU/μg, determined by LAL method.
复溶方法

Please use rapid thawing with running water to thaw the protein.
保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
运输条件

Shipping with dry ice.
文件资料

COA (147 KB)

产品使用指南 (1213 KB)

PGAM1 Protein, Human 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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目录号：
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