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  4. PHKG1 蛋白, Human (Active, sf9, GST)

PHKG1 蛋白, Human (Active, sf9, GST)

目录号: HY-P702665
产品使用指南 技术支持

PHKG1 是磷酸化酶 b 激酶 (PHK) 的催化亚基,通过磷酸化和激活糖原磷酸化酶,在糖原分解(糖原分解)的神经和激素调节中发挥关键作用。PHKG1 蛋白, Human (Active, sf9, GST) 是重组的 PHKG1 蛋白,由 Sf9 insect cells 表达,带有 GST 标签。PHKG1 蛋白, Human (Active, sf9, GST)

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描述

PHKG1 is the catalytic subunit of phosphorylase b kinase (PHK), which plays a key role in the neural and hormonal regulation of glycogenolysis (glycogen breakdown) by phosphorylating and activating glycogen phosphorylase. PHKG1 Protein, Human (Active, sf9, GST) is the recombinant human-derived PHKG1, expressed by Sf9 insect cells , with GST labeled tag. ,

研究背景

PHKG1(磷酸化酶激酶催化亚基 1)是磷酸化酶 b 激酶 (PHK) 的催化亚基,PHK 是参与糖原分解(糖原分解)的神经和激素调节的关键酶。其主要功能是磷酸化和激活糖原磷酸化酶,糖原磷酸化酶是糖原降解途径中的关键酶。PHKG1 已被证明对各种底物表现出体外磷酸化活性,包括 PYGM、TNNI3、MAPT/TAU、GAP43 和 NRGN/RC3,这表明 PHKG1 在调节糖原分解以外的过程中具有潜在作用,例如肌肉功能、微管动力学、和神经发育。参考文献强调了 PHKG1 在糖原代谢中的核心作用,并暗示其更广泛地参与多种底物的磷酸化,从而有助于细胞功能的复杂调节。

种属

Human

表达系统

Sf9 insect cells

标签

GST

蛋白编号

Q16816-1 (T2-Y387)

基因 ID

5260

同用名
PHKG1; phosphorylase kinase, gamma 1 (muscle); phosphorylase kinase, gamma 1 (muscle); phosphorylase b kinase gamma catalytic chain, skeletal muscle isoform; phosphorylase kinase gamma; phosphorylase kinase subunit gamma-1; EC 2.7.11.19
纯度

Greater than 80 % as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

PHKG1 Protein, Human (Active, sf9, GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
PHKG1 Protein, Human (Active, sf9, GST)
目录号:
HY-P702665
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