1. 重组蛋白
  2. Enzymes & Regulators
  3. Lyases (EC 4)
  4. PHS 蛋白, Human (His)

PHS 蛋白, Human (His)

目录号: HY-P71203
产品使用指南

PHS蛋白在四氢生物蝶呤生物合成中至关重要,具有阻碍7-蝶呤形成和促进醌-BH2的双重功能。它还充当 HNF1A 依赖性转录的共激活剂,影响 HNF1A 二聚化并增强其活性。PHS 蛋白, Human (His) 是重组的 PHS 蛋白,由 E. coli 表达,带有 N-6*His 标签。

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描述

PHS protein is crucial in tetrahydrobiopterin biosynthesis and has the dual function of hindering the formation of 7-pterin and promoting quinone-BH2. It also acts as a coactivator of HNF1A-dependent transcription, affecting HNF1A dimerization and enhancing its activity. PHS Protein, Human (His) is the recombinant human-derived PHS protein, expressed by E. coli , with N-6*His labeled tag.

研究背景

PHS 蛋白在四氢生物蝶呤生物合成中发挥着至关重要的作用,表明它参与了重要的细胞过程。它似乎表现出双重功能,阻碍 7-蝶呤的形成,同时加速醌类-BH2 的形成。除了在四氢生物蝶呤生物合成中的作用之外,PHS 还作为 HNF1A 依赖性转录的共激活剂,有助于基因表达的调节。值得注意的是,PHS 影响同源域蛋白 HNF1A 的二聚化,增强其转录活性。此外,它在 HNF1B 依赖性转录中充当共激活剂,展示了其在调节各种转录过程中的多功能性。这些多方面的功能强调了 PHS 在细胞通路和转录调控中的重要性。

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

P61457-1 (A2-T104)

基因 ID
蛋白结构
N-term
6*His
PHS (A2-T104)
Accession # P61457-1
C-term
同用名
Pterin-4-Alpha-Carbinolamine Dehydratase; PHS; 4-Alpha-Hydroxy-Tetrahydropterin Dehydratase; Dimerization Cofactor of Hepatocyte Nuclear Factor 1-Alpha; DCoH; Dimerization Cofactor of HNF1; Phenylalanine Hydroxylase-Stimulating Protein; Pterin Carbinolamine Dehy
氨基酸序列

AGKAHRLSAEERDQLLPNLRAVGWNELEGRDAIFKQFHFKDFNRAFGFMTRVALQAEKLDHHPEWFNVYNKVHITLSTHECAGLSERDINLASFIEQVAVSMT

分子量

Approximately 14.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

PHS Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HY-P71203
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