PIGR 蛋白, Mouse (HEK293, His)

PIGR proteins mediate the selective transcytosis of polymerized IgA and IgM across mucosal epithelial cells. It binds to these immunoglobulins at the basolateral surface, forming complexes that are transported intracellularly and secreted at the apical surface. PIGR Protein, Mouse (HEK293, His) is the recombinant mouse-derived PIGR protein, expressed by HEK293 , with C-His labeled tag. The total length of PIGR Protein, Mouse (HEK293, His) is 627 a.a., with molecular weight of 90-100 kDa.

PIGR 蛋白是聚合 IgA 和 IgM 跨粘膜上皮细胞选择性转胞吞作用的关键介质。在上皮细胞的基底外侧表面，它与聚合 IgA 和 IgM 结合，形成复合物，在细胞内运输，最终在顶端表面分泌。在整个转胞吞过程中，裂解事件将细胞外片段（称为分泌成分）与跨膜片段分开。PIGR 通过其 N 连接聚糖，确保分泌型 IgA (sIgA) 分子锚定在上皮表面的粘液上，有助于中和细胞外病原体。游离形式的 PIGR 还可以充当非特异性微生物清道夫，在防止病原体与上皮细胞相互作用方面发挥作用。

1.Measured by its binding ability in a functional ELISA. When Recombinant Mouse plgR is coated at 2 μg/mL (100 μL/well), the concentration of mouse IgM that produces 50% of the optimal binding response is 297.7 ng/mL.
2.Measured by its binding ability in a functional ELISA . Immobilized recombinant mouse PIGR at 5 μg/mL (100 μl/well) can bind mouse IgM with a linear range of 0.156-10 μg/mL.

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  Measured by its binding ability in a functional ELISA. When Recombinant Mouse plgR is coated at 2 μg/mL (100 μL/well), the concentration of mouse IgM that produces 50% of the optimal binding response is 297.7 ng/mL.

Mouse

HEK293

C-His

O70570 (K19-K645)

18703  [NCBI]

KSPIFGPQEVSSIEGDSVSITCYYPDTSVNRHTRKYWCRQGASGMCTTLISSNGYLSKEYSGRANLINFPENNTFVINIEQLTQDDTGSYKCGLGTSNRGLSFDVSLEVSQVPELPSDTHVYTKDIGRNVTIECPFKRENAPSKKSLCKKTNQSCELVIDSTEKVNPSYIGRAKLFMKGTDLTVFYVNISHLTHNDAGLYICQAGEGPSADKKNVDLQVLAPEPELLYKDLRSSVTFECDLGREVANEAKYLCRMNKETCDVIINTLGKRDPDFEGRILITPKDDNGRFSVLITGLRKEDAGHYQCGAHSSGLPQEGWPIQTWQLFVNEESTIPNRRSVVKGVTGGSVAIACPYNPKESSSLKYWCRWEGDGNGHCPVLVGTQAQVQEEYEGRLALFDQPGNGTYTVILNQLTTEDAGFYWCLTNGDSRWRTTIELQVAEATREPNLEVTPQNATAVLGETFTVSCHYPCKFYSQEKYWCKWSNKGCHILPSHDEGARQSSVSCDQSSQLVSMTLNPVSKEDEGWYWCGVKQGQTYGETTAIYIAVEERTRGSSHVNPTDANARAKVALEEEVVDSSISEKENKAIPNPGPFANEREIQNVGDQAQENRASGDAGSADGQSRSSSSK

90-100 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.