PIN1 蛋白, Human

The PIN1 protein is a peptidyl-prolyl cis/trans isomerase that complexly regulates multiple cellular processes by binding and isomerizing phosphorylated Ser/Thr-Pro motifs. This molecular switch induces conformational changes in phosphorylated proteins that affect mitosis, kinase activity, oncogene transactivation, centrosome amplification, and cell transformation. PIN1 Protein, Human is the recombinant human-derived PIN1 protein, expressed by E. coli , with tag free. The total length of PIN1 Protein, Human is 163 a.a., with molecular weight of ~18 kDa.

PIN1 蛋白是一种肽基脯氨酰顺/反异构酶 (PPIase)，通过结合特定磷酸化 Ser/Thr-Pro (pSer/Thr-Pro) 基序并使其异构化，复杂地参与多种细胞过程。这种分子开关诱导磷酸化蛋白质的构象变化，影响多种细胞途径。由于偏爱脯氨酸键 N 端的酸性残基，PIN1 通过与 NIMA 相互作用来调节有丝分裂，减弱其有丝分裂促进活性，并下调 BTK 的激酶活性。它反式激活癌基因，诱导中心体扩增、染色体不稳定和细胞转化。此外，PIN1 对于 RAF1 的去磷酸化和回收、结合并靶向 PML 和 BCL6 进行降解以及通过破坏 FBXW7 二聚化作为 JNK 级联的调节器至关重要。PIN1 的参与延伸到 DNA 修复过程，影响易错非同源末端连接 (NHEJ) 和无错同源重组 (HR) 之间的平衡。在 IL33 诱导的肺部炎症过程中，PIN1 催化磷酸化 IRAK3/IRAK-M 的顺反异构化，有助于 IRAK3 稳定、核易位以及树突状细胞中促炎基因的表达。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

Tag Free

Q13526 (M1-E163)

5300  [NCBI]

Approximately 18 kDa

Greater than 95% as determined by reducing SDS-PAGE

溶液

Supplied as a 0.2 μm filtered solution of 50 mM Tris, 10% Glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.