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  3. PITHD1 蛋白, Human (Myc, His)

PITHD1 蛋白, Human (Myc, His)

目录号: HY-P71631
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PITHD1 蛋白是细胞动力学的关键调节因子,特别是通过上调 RUNX1 表达来促进巨核细胞分化。它通过激活 RUNX1 基因的近端启动子并增强内部核糖体进入位点 (IRES) 元件的翻译活性来实现这一点。PITHD1 蛋白, Human (Myc, His) 是重组的 PITHD1 蛋白,由 E. coli 表达,带有 N-His, C-Myc 标签。

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描述

The PITHD1 protein is a key regulator of cell dynamics, specifically promoting megakaryocyte differentiation by upregulating RUNX1 expression. It does this by activating the proximal promoter of the RUNX1 gene and enhancing the translational activity of the internal ribosome entry site (IRES) element. PITHD1 Protein, Human (Myc, His) is the recombinant human-derived PITHD1 protein, expressed by E. coli , with N-His, C-Myc labeled tag.

研究背景

PITHD1 蛋白作为细胞动力学中的关键调节因子出现,特别是通过上调 RUNX1 的表达来促进巨核细胞分化。其调节影响延伸至 RUNX1 表达的调节,这是通过激活 RUNX1 基因的近端启动子和增强 RUNX1 基因内内部核糖体进入位点 (IRES) 元件的翻译活性来实现的。PITHD1 在这些过程中复杂的相互作用凸显了它在协调巨核细胞分化中的关键作用,强调了它在造血中的重要性。进一步探索 PITHD1 调节功能背后的详细机制,为了解其对细胞分化途径的更广泛影响提供了有希望的见解。

种属

Human

表达系统

E. coli

标签

N-His;C-Myc

蛋白编号

Q9GZP4-1 (M1-S211)

基因 ID

57095  [NCBI]

蛋白结构
N-term
10*His
PITHD1 (M1-S211)
Accession # Q9GZP4-1
C-term
同用名
PITHD1; C1orf128; AD039; HT014; PP603; PITH domain-containing protein 1
氨基酸序列

MSHGHSHGGGGCRCAAEREEPPEQRGLAYGLYLRIDLERLQCLNESREGSGRGVFKPWEERTDRSKFVESDADEELLFNIPFTGNVKLKGIIIMGEDDDSHPSEMRLYKNIPQMSFDDTEREPDQTFSLNRDLTGELEYATKISRFSNVYHLSIHISKNFGADTTKVFYIGLRGEWTELRRHEVTICNYEASANPADHRVHQVTPQTHFIS

分子量

Approximately 31.6 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

PITHD1 Protein, Human (Myc, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
PITHD1 Protein, Human (Myc, His)
目录号:
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