PK3C3 蛋白, Human (Sf9, His, GST)

The PI3KC3 protein is the catalytic subunit of the PI3K complex and mediates the formation of phosphatidylinositol 3-phosphate. PI3KC3-C1 initiates autophagosomes, whereas PI3KC3-C2 promotes autophagosome maturation and endocytosis. PK3C3 Protein, Human (Sf9, His, GST) is the recombinant human-derived PK3C3 protein, expressed by Sf9 insect cells , with N-8*His, N-GST labeled tag. The total length of PK3C3 Protein, Human (Sf9, His, GST) is 886 a.a., .

PK3C3 充当 PI3K 复合物的催化亚基，协调磷脂酰肌醇 3-磷酸的形成。PK3C3 的各种复杂形式在不同的膜运输途径中发挥着关键作用，其中 PI3KC3-C1 启动自噬体，PI3KC3-C2 促进自噬体的成熟和内吞作用。在 PI3KC3-C1 中，PK3C3 在囊泡出芽前积极促进内质网膜曲率的形成。此外，PK3C3 参与降解性内吞运输的调节，这对于胞质分裂中的脱离步骤至关重要，可能在 PI3KC3-C2 的背景下。此外，PK3C3 在溶酶体酶前体转运至溶酶体中发挥着至关重要的作用，并且是从早期内体到晚期内体的进展所必需的。值得注意的是，PK3C3 的激酶活性对于微生物感染期间 SARS 冠状病毒 (SARS-CoV-2) 的复制至关重要。

The activity of PK3C3 is verified by the ADP-Glo method: Incubate PK3C3 protein, ATP, and the substrate at room temperature for 40 minutes, then add the ADP-Glo reagent and read the luminescence signal using the chemiluminescence module of the microplate reader. When the protein concentration is 1.5 nM, the conversion rate is about 20%.

Human

Sf9 insect cells

N-8*His;N-GST

Q8NEB9 (G2-K887)

5289

Approximately 129.2 KDa.

Greater than 90% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 5% glycerol, 5 mM DTT, 0.1 M trehalose.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.