PKCz 蛋白, Human (Sf9, GST)

PKCz proteins are involved in multiple cellular processes, including mitotic signaling, cell proliferation, cell polarity, inflammatory responses, and maintenance of long-term potentiation (LTP). PKCz Protein, Human (Sf9) is the recombinant human-derived PKCz protein, expressed by Sf9 insect cells , with tag free. The total length of PKCz Protein, Human (Sf9) is 591 a.a., .

PKCz 是一种不依赖于钙和二酰甘油的丝氨酸/苏氨酸蛋白激酶，在细胞过程中发挥多方面的作用，在磷脂酰肌醇 3 激酶 (PI3K) 途径和丝裂原激活蛋白 (MAP) 激酶级联中发挥作用。PKCz 涉及多种功能，如 NF-kappa-B 激活、有丝分裂信号传导、细胞增殖、细胞极性、炎症反应和长时程增强 (LTP) 的维持，在其细胞功能中表现出多功能性。在各种情况下，它在 PI3K 下游发挥作用，独立激活 MAP2K1/MEK1-MAPK1/ERK2 信号级联，促进胰岛素依赖性 AKT3 激活，并参与 NF-kappa-B 的反式激活。此外，PKCz 在细胞极性的建立中发挥作用，刺激神经元分化，并通过参与 Th2 免疫反应参与过敏性气道炎症（哮喘）的发生。此外，PKCz 在 CA1 海马细胞的晚期突触长期增强阶段至关重要，并且与长期记忆维持相关。

The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device.

Human

Sf9 insect cells

N-GST

Q05513-1 (P2-V592)

5590

94.2 KDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, 150 mM NaCl, pH 7.5.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.