PLA2G2D 蛋白, Human (His-SUMO)

PLA2G2D protein is a secreted calcium-dependent phospholipase A2 that targets extracellular lipids and has anti-inflammatory and immunosuppressive functions.It hydrolyzes the fatty acyl group at the sn-2 position, preferentially hydrolyzing phosphatidylethanolamine and phosphatidylglycerol.PLA2G2D Protein, Human (His-SUMO) is the recombinant human-derived PLA2G2D protein, expressed by E.coli , with N-His, N-SUMO labeled tag.

PLA2G2D 是一种分泌型钙依赖性磷脂酶 A2，主要关注细胞外脂质，具有抗炎和免疫抑制功能。该蛋白质水解磷脂 sn-2 位脂肪酰基的酯键，显示出对磷脂酰乙醇胺和磷脂酰甘油的偏好超过磷脂酰胆碱。特别是在引流淋巴结中，它选择性地水解二酰基和烯基形式的磷脂酰乙醇胺，释放 omega-3 多不饱和脂肪酸，如二十碳五烯酸和二十二碳六烯酸。这些化合物充当合成抗炎脂质介质（称为分解素）的前体。在急性炎症的消退阶段，PLA2G2D 驱动二十二碳六烯酸衍生的消退素 D1 的合成，抑制树突状细胞活化和 T 辅助细胞 1 免疫反应。除了其催化活性外，PLA2G2D 通过与其酶功能无关的机制，促进调节性 T 细胞 (Treg) 的分化，并有助于维持免疫耐受。此外，它可能在细胞膜的脂质重塑中发挥作用，并参与对病原体清除至关重要的脂质介质的生成，通过直接水解细菌膜中的磷脂而对革兰氏阳性菌表现出杀菌活性。

Measured by its ability to hydrolyze 20 μΜ 1-Hexadecanoyl-2-(1-pyrene-decanoyl)-sn-glycero-3-phosphocholine. The specific activity is 8.049 pmol/min/μg that at room temperature in kinetic mode for 5 minutes.

Human

E. coli

N-His;N-SUMO

Q9UNK4 (I22-C145)

26279  [NCBI]

ILNLNKMVKQVTGKMPILSYWPYGCHCGLGGRGQPKDATDWCCQTHDCCYDHLKTQGCSIYKDYYRYNFSQGNIHCSDKGSWCEQQLCACDKEVAFCLKRNLDTYQKRLRFYWRPHCRGQTPGC

Approximately 30.5 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against solution in 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0 or 20 mM HEPES, 150 mM NaCl, pH 8.2, 0.2% CHAPS, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.