PF-4/CXCL4 蛋白, Mouse

Platelet factor 4 (PF4) is released during platelet aggregation and neutralizes the anticoagulant effect of heparin with a higher binding affinity than chondroitin 4-sulfate chains. In addition to its anticoagulant effects, PF4 induces neutrophil and monocyte chemotaxis, thereby promoting immune responses. PF-4/CXCL4 Protein, Mouse is the recombinant mouse-derived Platelet factor 4 protein, expressed by E. coli , with tag free.

血小板因子4（PF4）是血小板聚集过程中释放的蛋白质，对多种生理过程产生影响。与载体分子的 4-硫酸软骨素链相比，它对肝素表现出更高的结合亲和力，在中和肝素的抗凝作用中发挥着至关重要的作用。除了抗凝特性外，PF4 对中性粒细胞和单核细胞具有趋化作用，有助于免疫反应。此外，PF4 还可抑制内皮细胞增殖，表明其在调节血管过程中发挥作用。在结构上，PF4 形成同源四聚体，并与 TNFAIP6 相互作用，特别是与其 Link 结构域结合。这种复杂的相互作用凸显了 PF4 在止血、免疫反应和血管调节方面的多方面功能。

1.Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration of 10-100 ng/mL.
2.Measured by its ability to inhibit the FGF basic-dependent proliferation of HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is ≤0.2517 μg/mL, corresponding to a specific activity is ≥3.97×10³ U/mg.

• 
  Measured by its ability to inhibit the FGF basic-dependent proliferation of HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is 0.2517 μg/mL, corresponding to a specific activity is 3.97×10³ U/mg.

Mouse

E. coli

Tag Free

Q9Z126 (V30-S105)

56744  [NCBI]

VTSAGPEESDGDLSCVCVKTISSGIHLKHITSLEVIKAGRHCAVPQLIATLKNGRKICLDRQAPLYKKVIKKILES

Approximately 8.2-12 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm solution of PBS, pH 7.4 or 50 mM Tris-HCL, 300 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in sterile distilled water. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.