PLGF 蛋白, Human (N-His)

The PLGF-2 protein is a key growth factor for angiogenesis and endothelial cell growth, crucially stimulating cell proliferation and migration by binding to the FLT1/VEGFR-1 receptor. It coordinates the angiogenic process, regulates blood vessel growth, and exhibits additional binding capabilities to NRP1/neuropilin-1 and NRP2/neuropilin-2. PLGF Protein, Human (N-His) is the recombinant human-derived PLGF protein, expressed by E. coli , with N-6*His labeled tag. The total length of PLGF Protein, Human (N-His) is 131 a.a., with molecular weight of ~17 kDa.

PLGF-2 蛋白是一种在血管生成和内皮细胞生长中具有显着活性的生长因子，在刺激这些细胞的增殖和迁移中发挥着至关重要的作用。通过与 FLT1/VEGFR-1 受体结合，PLGF-2 协调血管生成过程并有助于调节血管生长。值得注意的是，异构体 PlGF-2 表现出额外的结合能力，以肝素依赖性方式与 NRP1/neuropilin-1 和 NRP2/neuropilin-2 形成相互作用。除了其血管生成功能外，PLGF-2 还促进肿瘤生长，表明其参与与癌症相关的病理性血管生成。在结构上，PLGF-2以二硫键连接的反向平行同二聚体的形式存在，并且可以进一步表现为与VEGFA/VEGF的异二聚体。同工型 PlGF-3 作为同二聚体和单体的存在增加了 PLGF 蛋白的复杂性，突出了它们在调节血管过程和肿瘤发生中的不同作用。

Measured in a cell proliferation assay using HUVEC Human umbilical vein endothelial cells. The ED₅₀ this effect is 20.22 ng/mL, corresponding to a specific activity is 4.95×10⁴ units/mg.

• 
  Measured in a cell proliferation assay using HUVEC Human umbilical vein endothelial cells. The ED₅₀ for this effect is 20.22 ng/mL, corresponding to a specific activity is 4.95×10⁴ units/mg.

Human

E. coli

N-6*His

P49763-2 (L19-R149)

5228  [NCBI]

LPAVPPQQWALSAGNGSSEVEVVPFQEVWGRSYCRALERLVDVVSEYPSEVEHMFSPSCVSLLRCTGCCGDENLHCVPVETANVTMQLLKIRSGDRPSYVELTFSQHVRCECRPLREKMKPERCGDAVPRR

Approximately 17 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, 200 mM arginine, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.