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  3. PMM2 蛋白, Human (His)

PMM2 蛋白, Human (His)

目录号: HY-P71021
产品使用指南

PMM2 蛋白对于 GDP-甘露糖和聚乙二醇-磷酸-甘露糖的合成至关重要,而 GDP-甘露糖和聚乙二醇-磷酸-甘露糖对于甘露糖基转移反应至关重要。其酶活性是含甘露糖糖复合物生物合成不可或缺的一部分,影响蛋白质糖基化并有助于细胞稳态。PMM2 蛋白, Human (His) 是重组的 PMM2 蛋白,由 E. coli 表达,带有 C-6*His 标签。

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描述

The PMM2 protein is essential for the synthesis of GDP-mannose and polyethylene glycol-phosphate-mannose, which are essential for the mannosyl transfer reaction. Its enzymatic activity is integral to the biosynthesis of mannose-containing glycoconjugates, affects protein glycosylation and contributes to cellular homeostasis. PMM2 Protein, Human (His) is the recombinant human-derived PMM2 protein, expressed by E. coli , with C-6*His labeled tag.

研究背景

PMM2 蛋白在 GDP-甘露糖和多甘醇-磷酸-甘露糖的合成中发挥着至关重要的作用,这对于许多关键的甘露糖基转移反应至关重要。其酶活性是含甘露糖糖复合物生物合成不可或缺的一部分,有助于各种细胞过程,例如蛋白质糖基化。PMM2 参与这些途径强调了其在细胞稳态和糖基化过程正常功能中的重要性,而糖基化过程对于合成具有重要生物学功能的各种糖缀合物至关重要。

种属

Human

表达系统

E. coli

标签

C-6*His

蛋白编号

O15305-1 (M1-S246)

基因 ID
蛋白结构
N-term
PMM2 (M1-S246)
Accession # O15305-1
6*His
C-term
同用名
Phosphomannomutase 2; PMM 2; PMM2
氨基酸序列

MAAPGPALCLFDVDGTLTAPRQKITKEMDDFLQKLRQKIKIGVVGGSDFEKVQEQLGNDVVEKYDYVFPENGLVAYKDGKLLCRQNIQSHLGEALIQDLINYCLSYIAKIKLPKKRGTFIEFRNGMLNVSPIGRSCSQEERIEFYELDKKENIRQKFVADLRKEFAGKGLTFSIGGQISFDVFPDGWDKRYCLRHVENDGYKTIYFFGDKTMPGGNDHEIFTDPRTMGYSVTAPEDTRRICELLFS

分子量

Approximately 29.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

PMM2 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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PMM2 Protein, Human (His)
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HY-P71021
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