PODXL 蛋白, Human (P.pastoris, His)

PODXL proteins coordinate multiple cellular functions, acting as anti-adhesion and pro-adhesion molecules. In podocytes, it maintains open filtration pathways through charge repulsion and promotes migration and cell-cell contacts in an integrin-dependent manner. PODXL Protein, Human (P.pastoris, His) is the recombinant human-derived PODXL protein, expressed by P. pastoris , with N-His labeled tag.

PODXL 蛋白在调节粘附、细胞形态和癌症进展中发挥多方面的作用。PODXL 作为一种抗粘附分子，通过电荷排斥在足细胞中的相邻足突之间维持开放的过滤通路。同时，它充当亲粘附分子，增强细胞对固定配体的粘附，促进迁移，并以整合素依赖性方式促进细胞与细胞的接触。PODXL 诱导顶端肌动蛋白依赖性微绒毛的形成，对于肾小管发生过程中初始上皮极化的建立和顶端管腔的形成至关重要。在癌症中，PODXL 通过与肌动蛋白结合蛋白 EZR 相互作用，影响 EZR 依赖性信号传导事件，从而导致 MAPK 和 PI3K 通路活性增加，从而促进细胞迁移和侵袭。其低聚状态各不相同，当与膜筏结合时作为单体存在，当整合到顶膜中时形成低聚物。PODXL 与 NHERF1、NHERF2 和 EZR 的相互作用凸显了其在细胞过程中的动态作用及其在不同细胞环境中的重要性。

Human

P. pastoris

N-His

O00592 (Q32-F458)

5420  [NCBI]

QNATQTTTDSSNKTAPTPASSVTIMATDTAQQSTVPTSKANEILASVKATTLGVSSDSPGTTTLAQQVSGPVNTTVARGGGSGNPTTTIESPKSTKSADTTTVATSTATAKPNTTSSQNGAEDTTNSGGKSSHSVTTDLTSTKAEHLTTPHPTSPLSPRQPTSTHPVATPTSSGHDHLMKISSSSSTVAIPGYTFTSPGMTTTLLETVFHHVSQAGLELLTSGDLPTLASQSAGITASSVISQRTQQTSSQMPASSTAPSSQETVQPTSPATALRTPTLPETMSSSPTAASTTHRYPKTPSPTVAHESNWAKCEDLETQTQSEKQLVLNLTGNTLCAGGASDEKLISLICRAVKATFNPAQDKCGIRLASVPGSQTVVVKEITIHTKLPAKDVYERLKDKWDELKEAGVSDMKLGDQGPPEEAEDRF

Approximately 120 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against solution in 20 mM Tris-HCl, 0.15 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.