PP1MG 蛋白, Human (His)

PP1MG Protein, a member of PPM family of serine/threonine phosphatases, has an important role in controlling cell cycle progression. PP1MG Proteinregulates the phosphorylation of SRSF3 in hepatocellular carcinoma (HCC) and contributes to the proliferation, invasion, and metastasis of HCC. PP1MG Protein forms a distinct holoenzyme complex with the PP2A regulatory subunit B56δ. B56δ‐PPM1G dephosphorylates α‐catenin at serine 641, which is necessary for the appropriate assembly of adherens junctions and the prevention of aberrant cell migration^[1][2]. PP1MG Protein, Human (His) is the recombinant human-derived PP1MG protein, expressed by E. coli , with C-6*His labeled tag. The total length of PP1MG Protein, Human (His) is 230 a.a., with molecular weight of ~30.0 kDa.

PPM 磷酸酶家族至少由 17 个成员组成。PPM 磷酸酶的活性依赖于 Mg2+ 或 Mn2+ 离子。B56δ 促进 PPM1G 重新定位到细胞质，在细胞质中磷酸酶可以访问一组离散的底物。α-连环蛋白是粘附连接的组成部分，作为细胞质中 PPM1G-B56 磷酸酶复合物的新底物。B56δ-PPM1G 使 α-连环蛋白在丝氨酸 641 处去磷酸化^[1]。
PPM1G 的过度表达促进 SRSF3 的去磷酸化，并改变与细胞周期、HCC 细胞转录调控相关的基因的选择性剪接模式。PPM1G 的敲低抑制了体内肿瘤的生长^[2]。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

C-6*His

O15355 (M317-D546)

5496  [NCBI]

MEGKEEPGSDSGTTAVVALIRGKQLIVANAGDSRCVVSEAGKALDMSYDHKPEDEVELARIKNAGGKVTMDGRVNGGLNLSRAIGDHFYKRNKNLPPEEQMISALPDIKVLTLTDDHEFMVIACDGIWNVMSSQEVVDFIQSKISQRDENGELRLLSSIVEELLDQCLAPDTSGDGTGCDNMTCIIICFKPRNTAELQPESGKRKLEEVLSTEGAEENGNSDKKKKAKRD

Approximately 30.0 kDa

Greater than 83% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 25 mM Tris-HCl, 1 mM DTT, 1 mM EDTA, 2 mM β-ME, 20% Glycerol, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.