1. 重组蛋白
  2. Receptor Proteins
  3. Nuclear Receptor Superfamily
  4. Peroxisome Proliferator-activated Receptor
  5. PPAR gamma
  6. PPAR gamma 蛋白, Human (His)

PPAR gamma LBD 蛋白, Human (His)是融合了 His 标签的人源的 PPAR-gamma 蛋白,E. coli 表达系统,约 32.6 kDa。PPAR gamma LBD 蛋白, Human (His) 可用于配体筛选试验、western blotting 和 ELISA 等。

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

PPAR gamma LBD Protein, Human (His) is a His-fused PPAR-gamma-LBD protein expressed by E. coli, approximately 32.6 kDa. PPAR gamma LBD Protein can be used in the ligand screening assays, western blotting, and ELISA, et al[1].

研究背景

过氧化物酶体增殖物激活受体(PPAR)属于核受体超家族,在调节膳食脂肪的储存和分解代谢中发挥重要作用。PPAR 包含三种亚型:PPARα、PPARβ 和 PPARγ[1]
许多天然存在的分子,例如;多不饱和脂肪酸,如花生四烯酸和花生四烯酸代谢物,例如;花生四烯酸和花生四烯酸代谢物等多不饱和脂肪酸可以结合并激活 PPARgamma[2]
LBD 是 PPARgamma 的配体结合结构域 (LBD),His 标记的 LBD 可用于配体筛选分析、蛋白质印迹和 ELISA 等的研究。

生物活性

PPAR gama immobilized on CM5 Chip can bind GW9662 with an affinity constant of 18.56 μM as determined in SPR assay.

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

P37231-1 (D238-D503)

基因 ID
蛋白结构
N-term
6*His
PPAR gamma (D238-D503)
Accession # P37231-1
C-term
同用名
PPAR-γ-LBD
氨基酸序列

MGSSHHHHHHSSGLVPRGSHDLRALAKHLYDSYIKSFPLTKAKARAILTGKTTDKSPFVIYDMNSLMMGEDKIKFKHITPLQEQSKEVAIRIFQGCQFRSVEAVQEITEYAKSIPGFVNLDLNDQVTLLKYGVHEIIYTMLASLMNKDGVLISEGQGFMTREFLKSLRKPFGDFMEPKFEFAVKFNALELDDSDLAIFIAVIILSGDRPGLLNVKPIEDIQDNLLQALELQLKLNHPESSQLFAKLLQKMTDLRQIVTEHVQLLQVIKKTETDMSLHPLLQEIYKD

分子量

Approximately 30-32.6 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0 or PBS, pH 7.4, 8% trehalose.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
PPAR gamma Protein, Human (His)
目录号:
HY-P7999
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