PPAR gamma 蛋白, Human (His)

PPAR gamma LBD Protein, Human (His) is a His-fused PPAR-gamma-LBD protein expressed by E. coli, approximately 32.6 kDa. PPAR gamma LBD Protein can be used in the ligand screening assays, western blotting, and ELISA, et al^[1].

过氧化物酶体增殖物激活受体（PPAR）属于核受体超家族，在调节膳食脂肪的储存和分解代谢中发挥重要作用。PPAR 包含三种亚型：PPARα、PPARβ 和 PPARγ^[1]。
许多天然存在的分子，例如；多不饱和脂肪酸，如花生四烯酸和花生四烯酸代谢物，例如；花生四烯酸和花生四烯酸代谢物等多不饱和脂肪酸可以结合并激活 PPARgamma^[2]。
LBD 是 PPARgamma 的配体结合结构域 (LBD)，His 标记的 LBD 可用于配体筛选分析、蛋白质印迹和 ELISA 等的研究。

PPAR gama immobilized on CM5 Chip can bind GW9662 with an affinity constant of 18.56 μM as determined in SPR assay.

Human

E. coli

N-6*His

P37231-1 (D238-D503)

5468  [NCBI]

MGSSHHHHHHSSGLVPRGSHDLRALAKHLYDSYIKSFPLTKAKARAILTGKTTDKSPFVIYDMNSLMMGEDKIKFKHITPLQEQSKEVAIRIFQGCQFRSVEAVQEITEYAKSIPGFVNLDLNDQVTLLKYGVHEIIYTMLASLMNKDGVLISEGQGFMTREFLKSLRKPFGDFMEPKFEFAVKFNALELDDSDLAIFIAVIILSGDRPGLLNVKPIEDIQDNLLQALELQLKLNHPESSQLFAKLLQKMTDLRQIVTEHVQLLQVIKKTETDMSLHPLLQEIYKD

Approximately 30-32.6 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Changying Yu, et al.Binding analyses between Human PPARgamma-LBD and ligands. Eur J Biochem. 2004 Jan;271(2):386-97  [Content Brief]

  [2]. Jun Young Jang, et al. Structural Basis for the Enhanced Anti-Diabetic Efficacy of Lobeglitazone on PPARγ. Sci Rep. 2018 Jan 8;8(1):31.  [Content Brief]