PPAR gamma 蛋白, Human (His)

PPAR gamma LBD Protein, Human (His) is a His-fused PPAR-gamma-LBD protein expressed by E. coli, approximately 32.6 kDa. PPAR gamma LBD Protein can be used in the ligand screening assays, western blotting, and ELISA, et al^[1].

过氧化物酶体增殖物激活受体（PPAR）属于核受体超家族，在调节膳食脂肪的储存和分解代谢中发挥重要作用。PPAR 包含三种亚型：PPARα、PPARβ 和 PPARγ^[1]。
许多天然存在的分子，例如；多不饱和脂肪酸，如花生四烯酸和花生四烯酸代谢物，例如；花生四烯酸和花生四烯酸代谢物等多不饱和脂肪酸可以结合并激活 PPARgamma^[2]。
LBD 是 PPARgamma 的配体结合结构域 (LBD)，His 标记的 LBD 可用于配体筛选分析、蛋白质印迹和 ELISA 等的研究。

Human

E. coli

N-6*His

P37231-1 (D238-D503)

5468  [NCBI]

MGSSHHHHHHSSGLVPRGSHDLRALAKHLYDSYIKSFPLTKAKARAILTGKTTDKSPFVIYDMNSLMMGEDKIKFKHITPLQEQSKEVAIRIFQGCQFRSVEAVQEITEYAKSIPGFVNLDLNDQVTLLKYGVHEIIYTMLASLMNKDGVLISEGQGFMTREFLKSLRKPFGDFMEPKFEFAVKFNALELDDSDLAIFIAVIILSGDRPGLLNVKPIEDIQDNLLQALELQLKLNHPESSQLFAKLLQKMTDLRQIVTEHVQLLQVIKKTETDMSLHPLLQEIYKD

Approximately 32.6 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Changying Yu, et al.Binding analyses between Human PPARgamma-LBD and ligands. Eur J Biochem. 2004 Jan;271(2):386-97  [Content Brief]

  [2]. Jun Young Jang, et al. Structural Basis for the Enhanced Anti-Diabetic Efficacy of Lobeglitazone on PPARγ. Sci Rep. 2018 Jan 8;8(1):31.  [Content Brief]