PPIH 蛋白, Human (His)

The PPIH protein is a peptidyl prolyl cis-trans isomerase (PPIase) that catalyzes the isomerization of prolyl imide peptide bonds and may contribute to protein folding. It plays a crucial role in pre-mRNA splicing and contributes to the formation of the U4/U5/U6 tri-snRNP complex in spliceosome assembly. PPIH Protein, Human (His) is the recombinant human-derived PPIH protein, expressed by E. coli , with N-6*His labeled tag.

PPIH 蛋白充当肽基-脯氨酰顺反异构酶 (PPIase)，促进寡肽中脯氨酸酰亚胺肽键的顺反异构化，并可能有助于蛋白质折叠。除了在蛋白质折叠中的作用之外，PPIH 还积极参与前 mRNA 剪接，这表明 PPIH 参与了剪接体组装的复杂过程。具体来说，它可能有助于 U4/U5/U6 tri-snRNP 复合物的形成，该复合物是剪接体的基本组成部分。这种多功能作用意味着 PPIH 可能充当伴侣，强调其在蛋白质折叠和与 mRNA 剪接相关的重要细胞过程的调节中的多功能参与。需要进一步探索以阐明 PPIH 在蛋白质稳态和前 mRNA 剪接中发挥其功能的具体分子机制和细胞环境。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

O43447-1 (M1-M177)

10465  [NCBI]

MAVANSSPVNPVVFFDVSIGGQEVGRMKIELFADVVPKTAENFRQFCTGEFRKDGVPIGYKGSTFHRVIKDFMIQGGDFVNGDGTGVASIYRGPFADENFKLRHSAPGLLSMANSGPSTNGCQFFITCSKCDWLDGKHVVFGKIIDGLLVMRKIENVPTGPNNKPKLPVVISQCGEM

Approximately 24.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.