PPIL2 蛋白, Human (His)

PPIL2 exhibits ubiquitin-protein ligase activity, acting as an E3 ligase or ubiquitin-ubiquitin ligase, promoting “Lys-48”-linked polyubiquitination for proteasomal degradation of substrates.It acts as a chaperone to help transport BSG/Basigin to the cell membrane.PPIL2 Protein, Human (His) is the recombinant human-derived PPIL2 protein, expressed by E.coli , with N-His labeled tag.

PPIL2 以其泛素蛋白连接酶活性而闻名，它同时具有 E3 泛素蛋白连接酶和泛素-泛素连接酶的功能，促进底物上泛素链的延长。通过介导“Lys-48”连接的多聚泛素化，它有可能靶向蛋白质进行蛋白酶体降解。此外，PPIL2 可以作为伴侣，有助于将 BSG/Basigin 等蛋白质转运到细胞膜。尽管具有泛素连接酶功能，PPIL2 很可能是一种无活性的肽基脯氨酰顺反异构酶。作为小剪接体的一部分，PPIL2 在前体 mRNA 中 U12 型内含子的剪接中发挥作用，表明它参与了 RNA 剪接的复杂过程。

Human

E. coli

N-His

Q13356-2 (G280-P457)

23759  [NCBI]

GYVRLHTNKGDLNLELHCDLTPKTCENFIRLCKKHYYDGTIFHRSIRNFVIQGGDPTGTGTGGESYWGKPFKDEFRPNLSHTGRGILSMANSGPNSNRSQFFITFRSCAYLDKKHTIFGRVVGGFDVLTAMENVESDPKTDRPKEEIRIDATTVFVDPYEEADAQIAQERKTQLKVAP

Approximately 24 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.