PPM1A 蛋白, Mouse (His)

PPM1A is a multifunctional enzyme whose enzymatic activity exhibits broad specificity. It acts as a negative regulator of TGF-β signaling by dephosphorylating SMAD2 and SMAD3, leading to their dissociation from SMAD4, nuclear export of SMAD, and termination of TGF-β-mediated signaling. PPM1A Protein, Mouse (His) is the recombinant mouse-derived PPM1A protein, expressed by E. coli , with C-His labeled tag.

蛋白磷酸酶，Mg2+/Mn2+ 依赖性 1A (PPM1A) 是一种具有广泛特异性的酶。它通过使 SMAD2 和 SMAD3 去磷酸化，充当 TGF-β 信号传导的负调节因子，导致它们从 SMAD4 解离并随后进行核输出。这种酶活性最终终止 TGF-β 介导的信号通路。此外，PPM1A 使 PRKAA1 和 PRKAA2 去磷酸化，有助于调节与 AMP 激活蛋白激酶相关的细胞过程。此外，PPM1A 通过去磷酸化和灭活 IKBKB/IKKB，在终止 TNF-α 介导的 NF-κ-B 激活中发挥关键作用。PPM1A 的多种底物特异性强调了其在调节关键信号传导途径中的重要性，强调了其在细胞对 TGF-β 和 TNF-α 信号传导反应中的调节功能（参考：提供的信息）。

Measured by its ability to dephosphorylate the peptide substrate, DLDVPIPGRFDRRVS(PO3)VAAE. The specific activity is 450.99 nmol/min/mg, as measured under the described conditions.

Mouse

E. coli

C-6*His

P49443/NP_032936.1 (M1-W382)

19042  [NCBI]

MGAFLDKPKMEKHNAQGQGNGLRYGLSSMQGWRVEMEDAHTAVIGLPSGLETWSFFAVYDGHAGSQVAKYCCEHLLDHITNNQDFRGSAGAPSVENVKNGIRTGFLEIDEHMRVMSEKKHGADRSGSTAVGVLISPQHTYFINCGDSRGLLCRNRKVHFFTQDHKPSNPLEKERIQNAGGSVMIQRVNGSLAVSRALGDFDYKCVHGKGPTEQLVSPEPEVHDIERSEEDDQFIILACDGIWDVMGNEELCDFVRSRLEVTDDLEKVCNEVVDTCLYKGSRDNMSVILICFPSAPKVSAEAVKKEAELDKYLESRVEEIIKKQVEGVPDLVHVMRTLASENIPSLPPGGELASKRNVIEAVYNRLNPYKNDDTDSASTDDMW

Approximately 44 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.