1. 重组蛋白
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. PpSQ1_00405 蛋白, Pseudomonas putida (His, Strep)

PpSQ1_00405 蛋白, Pseudomonas putida (His, Strep)

目录号: HY-P702158
产品使用指南

PpSQ1_00405 蛋白促进磺基喹诺糖氧化为 6-脱氧-6-磺基-D-葡萄糖酸-1,5-内酯,显示出对 NAD(+) 作为电子受体的显着偏好。这种酶活性是磺基喹诺糖 (SQ) 降解途径的重要组成部分,使恶臭假单胞菌菌株 SQ1 利用 SQ 作为其生长的唯一碳和能源。PpSQ1_00405 蛋白, Pseudomonas putida (His, Strep) 是重组的 PpSQ1_00405 蛋白,由 E. coli 表达,带有 N-Strep, N-6*His 标签。PpSQ1_00405 蛋白, Pseudomonas putida (His, Strep) 全长 259 个氨基酸。

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描述

The PpSQ1_00405 protein promotes the oxidation of sulfoquinolose to 6-deoxy-6-sulfo-D-glucono-1,5-lactone, showing a significant preference for NAD(+) as the electron acceptor. This enzymatic activity is an important component of the sulfoquinolose (SQ) degradation pathway, allowing P. putida strain SQ1 to utilize SQ as the sole carbon and energy source for its growth. PpSQ1_00405 Protein, Pseudomonas putida (His, Strep) is the recombinant PpSQ1_00405 protein, expressed by E. coli , with N-Strep, N-6*His labeled tag. The total length of PpSQ1_00405 Protein, Pseudomonas putida (His, Strep) is 259 a.a., .

研究背景

PpSQ1_00405 是一种在恶臭假单胞菌 SQ1 的磺基奎诺糖 (SQ) 降解途径中发挥关键作用的酶,使细菌能够利用 SQ 作为其生长的唯一碳和能源。该蛋白质催化磺基奎诺糖的氧化,将其转化为 6-脱氧-6-磺基-D-葡萄糖酸-1,5-内酯。值得注意的是,PpSQ1_00405 在该氧化反应中表现出对 NAD(+) 作为电子受体的强烈偏好。该酶有效催化磺基奎诺糖转化的能力强调了其在 SQ 微生物代谢中的重要性,揭示了细菌利用这种硫脂作为维持生长和能量产生的营养源的途径(改编自所提供的通道)。

种属

Others

表达系统

E. coli

标签

N-Strep;N-6*His

蛋白编号

P0DOV5 (N2-Q260)

基因 ID

/

蛋白结构
N-term
Strep-6*His
PpSQ1_00405 (N2-Q260)
Accession # P0DOV5
C-term
同用名
PpSQ1_00405; Sulfoquinovose 1-dehydrogenase; SQ dehydrogenase
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

PpSQ1_00405 Protein, Pseudomonas putida (His, Strep) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
PpSQ1_00405 Protein, Pseudomonas putida (His, Strep)
目录号:
HY-P702158
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