PSAP/Prosaposin 蛋白, Mouse (HEK293, His)

PSAP/Prosaposin protein is a myelin trophic factor and neurotrophic factor that acts through GPR37 and GPR37L1 receptors.This triggers ligand-mediated internalization, initiating ERK phosphorylation signaling.PSAP/Prosaposin Protein, Mouse (HEK293, His) is the recombinant mouse-derived PSAP/Prosaposin protein, expressed by HEK293 , with C-His labeled tag.

PSAP/Prosaposin 蛋白作为髓鞘营养因子和神经营养因子，通过 G 蛋白偶联受体 GPR37 和 GPR37L1 发挥其作用。这种相互作用触发配体介导的内化，随后启动 ERK 磷酸化信号传导。此外，该蛋白质在鞘脂代谢中表现出调节能力，因为 Saposin-A 和 Saposin-C 均促进 β-葡萄糖神经酰胺酶 (EC 3.2.1.45) 水解葡萄糖神经酰胺，并促进 β-半乳糖神经酰胺酶 (EC 3.2.1.46) 水解半乳糖神经酰胺。值得注意的是，Saposin-C 的作用模式涉及与酶和酸性脂质形成活化复合物，而不是溶解底物，这凸显了其在细胞过程中的微妙作用。

Mouse

HEK293

C-His

Q61207/NP_035309.3 (S17-N557)

19156  [NCBI]

SPVQDPKTCSGGSAVLCRDVKTAVDCGAVKHCQQMVWSKPTAKSLPCDICKTVVTEAGNLLKDNATQEEILHYLEKTCEWIHDSSLSASCKEVVDSYLPVILDMIKGEMSNPGEVCSALNLCQSLQEYLAEQNQKQLESNKIPEVDMARVVAPFMSNIPLLLYPQDHPRSQPQPKANEDVCQDCMKLVSDVQTAVKTNSSFIQGFVDHVKEDCDRLGPGVSDICKNYVDQYSEVCVQMLMHMQDQQPKEICVLAGFCNEVKRVPMKTLVPATETIKNILPALEMMDPYEQNLVQAHNVILCQTCQFVMNKFSELIVNNATEELLVKGLSNACALLPDPARTKCQEVVGTFGPSLLDIFIHEVNPSSLCGVIGLCAARPELVEALEQPAPAIVSALLKEPTPPKQPAQPKQSALPAHVPPQKNGGFCEVCKKLVLYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDDFVAEYEPLLLEILVEVMDPGFVCSKIGVCPSAYKLLLGTEKCVWGPSYWCQNMETAARCNAVDHCKRHVWN

Approximately 61.2 kDa.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4 or PBS, pH 7.4

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.