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  3. PYHIN1 蛋白, Human (His, Strep)

PYHIN1 蛋白, Human (His, Strep)

目录号: HY-P701995
产品使用指南

PYHIN1 蛋白是乳腺癌细胞中 IFN 肿瘤抑制活性的主要介质,可促进 MDM2 的泛素化和降解,从而导致 p53/TP53 稳定。它还促进 HDAC1 的泛素化和降解,增强 maspin 的表达并削弱癌细胞的侵袭活性。PYHIN1 在这些调节过程中与 MDM2 相互作用。PYHIN1 蛋白, Human (His, Strep) 是重组的 PYHIN1 蛋白,由 E. coli 表达,带有 N-Strep, N-6*His 标签。PYHIN1 蛋白, Human (His, Strep) 全长 492 个氨基酸。

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描述

PYHIN1 Protein, a major mediator of IFN's tumor suppressor activity in breast cancer cells, facilitates the ubiquitination and degradation of MDM2, leading to p53/TP53 stabilization. It also promotes the ubiquitination and degradation of HDAC1, enhancing maspin expression and impairing the invasive activity of cancer cells. PYHIN1 interacts with MDM2 in these regulatory processes. PYHIN1 Protein, Human (His, Strep) is the recombinant human-derived PYHIN1 protein, expressed by E. coli , with N-Strep, N-6*His labeled tag. The total length of PYHIN1 Protein, Human (His, Strep) is 492 a.a., .

研究背景

PYHIN1 蛋白作为乳腺癌细胞中的关键介质而出现,在放大 IFN 的肿瘤抑制活性方面发挥着关键作用。它促进 MDM2 的泛素化和随后的降解,从而稳定 p53/TP53。此外,PYHIN1 促进 HDAC1 泛素化和随后的降解,导致 maspin 表达增加并减少癌细胞的侵袭活性。它与 MDM2 的相互作用强调了它在对癌细胞行为至关重要的调控途径中的复杂参与。

种属

Human

表达系统

E. coli

标签

N-Strep;N-6*His;C-Flag

蛋白编号

Q6K0P9 (M1-P492)

基因 ID

149628

蛋白结构
N-term
6*His-Strep
PYHIN1 (M1-P492)
Accession # Q6K0P9
Flag
C-term
同用名
PYHIN1; Pyrin and HIN domain-containing protein 1; Interferon-inducible protein X
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

PYHIN1 Protein, Human (His, Strep) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
PYHIN1 Protein, Human (His, Strep)
目录号:
HY-P701995
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