RANKL/TNFSF11 蛋白, Mouse (CHO, His)

RANKL/TNFSF11 Protein, Mouse (CHO) is a TNF-related activation-induced cytokine produced in CHO. Mouse and human RANK Ligand share 85% amino acid identity. RANK L/TNFSF11 Protein, Mouse (CHO, His) induces the activation of the c-jun N terminal kinase, enhances T cell growth and dendritic cell function, induces osteoclastogenesis, and lymph node organogenesis.

NF-κB配体受体激活剂(RANKL)、其细胞受体、NF-κB受体激活剂(RANK)和诱饵受体骨保护素(OPG)构成了一种新型细胞因子系统。成骨细胞谱系细胞和活化的T淋巴细胞产生的RANKL是破骨细胞形成、融合、激活和存活的重要因素，从而导致骨吸收和骨丢失。RANKL 激活其位于破骨细胞和树突状细胞上的特异性受体 RANK，其信号级联涉及刺激 c-jun、NF-κB 和丝氨酸/苏氨酸激酶 PKB/Akt 通路^[1]。

Measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells. The ED₅₀ is 0.2397 ng/mL, corresponding to a specific activity of 4.172 × 10⁶ units/mg.

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  Measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells. The ED₅₀ is <0.2397 ng/mL, corresponding to a specific activity of >4.172 × 10⁶ units/mg.

Mouse

CHO

C-6*His

O35235-1 (R72-D316)

21943  [NCBI]

RAQMDPNRISEDSTHCFYRILRLHENADLQDSTLESEDTLPDSCRRMKQAFQGAVQKELQHIVGPQRFSGAPAMMEGSWLDVAQRGKPEAQPFAHLTINAASIPSGSHKVTLSSWYHDRGWAKISNMTLSNGKLRVNQDGFYYLYANICFRHHETSGSVPTDYLQLMVYVVKTSIKIPSSHNLMKGGSTKNWSGNSEFHFYSINVGGFFKLRAGEEISIQVSNPSLLDPDQDATYFGAFKVQDID

Approximately 34.5 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.8.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.