RANTES/CCL5 蛋白, Human (Biotinylated, His-Avi)

RANTES/CCL5 protein attracts monocytes, memory T-helper cells, and eosinophils, releases histamine, and activates eosinophils. It interacts with chemokine receptors CCR1, CCR3, CCR4, and CCR5. It inhibits HIV and may promote neuron survival and insulin secretion via GPR75. RANTES/CCL5 Protein, Human (Biotinylated, His-Avi) is the recombinant human-derived RANTES/CCL5 protein, expressed by E. coli , with N-Avi, N-His labeled tag. The total length of RANTES/CCL5 Protein, Human (Biotinylated, His-Avi) is 68 a.a., with molecular weight of 10.89 kDa.

RANTES/CCL5 蛋白是一种化学引诱剂，在吸引血液单核细胞、记忆 T 辅助细胞和嗜酸性粒细胞方面发挥作用。它还导致嗜碱性粒细胞释放组胺并激活嗜酸性粒细胞。RANTES 可以激活多种趋化因子受体，包括 CCR1、CCR3、CCR4 和 CCR5。已知它是 CD8+ T 细胞产生的主要 HIV 抑制因子之一。重组 RANTES 蛋白已显示出以剂量依赖性方式抑制不同毒株的 HIV-1、HIV-2 和猿猴免疫缺陷病毒 (SIV)。RANTES 有不同的加工形式，例如 RANTES(3-68)，它作为天然趋化性抑制剂，与其他加工形式 RANTES(4-68) 和 RANTES( 1-68）。RANTES 还可以作为 G 蛋白偶联受体 GPR75 的激动剂，刺激三磷酸肌醇的产生和钙动员。研究表明，RANTES 与 GPR75 可能通过激活涉及 PI3、Akt 和 MAP 激酶的下游信号通路在神经元存活中发挥作用。此外，通过激活 GPR75，RANTES 还可能有助于胰岛细胞分泌胰岛素。RANTES 可以与其他趋化因子形成同源和异源寡聚体，并与棕色狗蜱 evasin-4 相互作用。

1.Immobilized Anti-CCL5 Antibody, hFc Tag at 1 μg/mL (100 μl/well) on the plate. Dose response curve for Biotinylated Human CCL5, His Tag with the EC₅₀ of ≤20 ng/mL determined by ELISA.
2.Immobilized Biotinylated Human CCL5, His Tag at 1μg/ml (100μl/Well) on streptavidin (5 μg/ml) precoated plate. Dose response curve for Anti CCL5 Antibody, hFc Tag with the EC₅₀ of 5.6 ng/mL determined by ELISA.

Human

E. coli

N-His;N-Avi

P13501 (S24-S91)

6352  [NCBI]

10.89 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, 0.3%SKL, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.