RBM14 蛋白, Human (His-SUMO)

The RBM14 protein has different isoforms with multiple functions. Isoform 1 enhances transcription as a nuclear receptor coactivator, interacting with NCOA6 and CITED1. RBM14 Protein, Human (His-SUMO) is the recombinant human-derived RBM14 protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag. The total length of RBM14 Protein, Human (His-SUMO) is 669 a.a., with molecular weight of ~85.5 kDa.

RBM14 蛋白根据其亚型表现出不同的功能作用。同工型 1 作为核受体共激活剂，通过与 NCOA6 和 CITED1 等共激活剂相互作用来增强转录。相比之下，Isoform 2 作为转录阻遏蛋白发挥作用，调节共激活因子（包括 Isoform 1、NCOA6 和 CITED1）的活性。值得注意的是，RBM14 参与中心粒生物发生的调节，它抑制异常中心粒蛋白复合物的形成，确保有丝分裂纺锤体的完整性。此外，RBM14 通过组装成 HDP-RNP 复合物，作为 IRF3 磷酸化的平台并通过 cGAS-STING 途径激活先天免疫反应，在 DNA 病毒介导的先天免疫反应中发挥着至关重要的作用。与各种蛋白质（包括 NCOA6、CITED1、XRCC5/KU86、SS18 同工型、STIL 和 γ-微管蛋白）的相互作用强调了其参与多种细胞过程和信号传导途径。此外，RBM14 融入 HDP-RNP 复合物凸显了其在协调针对 DNA 病毒的先天免疫反应中的作用。

Human

E. coli

N-SUMO;N-6*His

Q96PK6 (M1-M669)

10432  [NCBI]

MKIFVGNVDGADTTPEELAALFAPYGTVMSCAVMKQFAFVHMRENAGALRAIEALHGHELRPGRALVVEMSRPRPLNTWKIFVGNVSAACTSQELRSLFERRGRVIECDVVKDYAFVHMEKEADAKAAIAQLNGKEVKGKRINVELSTKGQKKGPGLAVQSGDKTKKPGAGDTAFPGTGGFSATFDYQQAFGNSTGGFDGQARQPTPPFFGRDRSPLRRSPPRASYVAPLTAQPATYRAQPSVSLGAAYRAQPSASLGVGYRTQPMTAQAASYRAQPSVSLGAPYRGQLASPSSQSAAASSLGPYGGAQPSASALSSYGGQAAAASSLNSYGAQGSSLASYGNQPSSYGAQAASSYGVRAAASSYNTQGAASSLGSYGAQAASYGAQSAASSLAYGAQAASYNAQPSASYNAQSAPYAAQQAASYSSQPAAYVAQPATAAAYASQPAAYAAQATTPMAGSYGAQPVVQTQLNSYGAQASMGLSGSYGAQSAAAATGSYGAAAAYGAQPSATLAAPYRTQSSASLAASYAAQQHPQAAASYRGQPGNAYDGAGQPSAAYLSMSQGAVANANSTPPPYERTRLSPPRASYDDPYKKAVAMSKRYGSDRRLAELSDYRRLSESQLSFRRSPTKSSLDYRRLPDAHSDYARYSGSYNDYLRAAQMHSGYQRRM

Approximately 85.5 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.