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  3. RBP7 蛋白, Human (His)

RBP7 蛋白, Human (His)

目录号: HY-P71251A
COA 产品使用指南

RBP7蛋白是CRBP家族的一员,参与维生素A的稳定性和代谢。它与全反式视黄醇结合,但与其他CRBP相比亲和力较低。RBP7 蛋白在脂肪、脾脏和其他组织中表达,表明可能具有组织特异性功能。RBP7 蛋白, Human (His) 是重组的 RBP7 蛋白,由 E. coli 表达,带有 N-6*His 标签。

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描述

RBP7 protein, a member of the CRBP family, is involved in the stability and metabolism of vitamin A. It binds to all-trans-retinol but with lower affinity compared to other CRBPs. RBP7 protein is expressed in fat, spleen, and other tissues, indicating possible tissue-specific functions. RBP7 Protein, Human (His) is the recombinant human-derived RBP7 protein, expressed by E. coli , with N-6*His labeled tag.

研究背景

RBP7 蛋白属于细胞视黄醇结合蛋白 (CRBP) 家族,在维生素 A 的稳定性和代谢中发挥着至关重要的作用。它对全反式视黄醇具有结合亲和力,并且与其他 CRBP 具有结构相似性。其与视黄醇的结合亲和力相对较低。除了其一般功能外,该蛋白在脂肪 (RPKM 138.2)、脾 (RPKM 25.4) 和其他七个组织中表现出偏向表达,表明潜在的组织特异性作用。

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

Q96R05/NP_443192.1 (P2-A134)

基因 ID
蛋白结构
N-term
6*His
RBP7 (P2-A134)
Accession # Q96R05/NP_443192.1
C-term
同用名
Retinoid-binding protein 7; Cellular retinoic acid-binding protein 4; CRABP4; CRBP4; Cellular retinoic acid-binding protein IV; CRABP-IV; RBP7
氨基酸序列

PADLSGTWTLLSSDNFEGYMLALGIDFATRKIAKLLKPQKVIEQNGDSFTIHTNSSLRNYFVKFKVGEEFDEDNRGLDNRKCKSLVIWDNDRLTCIQKGEKKNRGWTHWIEGDKLHLEMFCEGQVCKQTFQRA

分子量

Approximately 16 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, 300 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

RBP7 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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RBP7 Protein, Human (His)
目录号:
HY-P71251A
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