RCVRN 蛋白, Human (P.pastoris, His)

RCVRN protein, a calcium sensor, regulates phototransduction in cone and rod photoreceptor cells, enhancing cone photoreceptor light sensitivity in dark conditions. In low light, it extends RHO/rhodopsin activation in rods by inhibiting GRK1-mediated phosphorylation. RCVRN contributes to scotopic vision, improving signal transfer from rods to rod bipolar cells, enhancing vision in low light. It boosts rod photoreceptor sensitivity in dim light, aiding motion detection. Existing as a homodimer, RCVRN undergoes disulfide-linked dimerization in intense illumination and may form a Ca(2+)-dependent complex with RHO and GRK1, preventing their interaction. RCVRN Protein, Human (P.pastoris, His) is the recombinant human-derived RCVRN protein, expressed by P. pastoris, with N-His labeled tag. The total length of RCVRN Protein, Human (P.pastoris, His) is 199 a.a., with molecular weight of ~25.0 kDa.

RCVRN 蛋白作为钙传感器，复杂地参与调节视锥细胞和视杆细胞的光转导。它在调节视锥细胞的光敏感性方面发挥着至关重要的作用，特别是在黑暗和昏暗的条件下。为了响应弱光引起的 Ca(2+) 水平升高，RCVRN 通过结合并抑制 GRK1 介导的 RHO/视紫红质磷酸化，延长视杆细胞中 RHO/视紫红质的激活。这种蛋白质有助于暗视觉，通过促进视杆细胞感光器和视杆细胞双极细胞之间的信号传递来增强弱光条件下的视力。此外，RCVRN 还提高了视杆细胞感光器在弱光下的灵敏度，调节有助于检测强光下变化和运动的反应。RCVRN 作为同二聚体存在，会经历二硫键连接的二聚化，这是由长时间强光照触发的过程。此外，RCVRN可能以Ca(2+)依赖性方式与RHO和GRK1形成复合物，从而阻止GRK1和RHO之间的相互作用。

Human

P. pastoris

N-His

P35243 (2G-200A)

5957  [NCBI]

GNSKSGALSKEILEELQLNTKFSEEELCSWYQSFLKDCPTGRITQQQFQSIYAKFFPDTDPKAYAQHVFRSFDSNLDGTLDFKEYVIALHMTTAGKTNQKLEWAFSLYDVDGNGTISKNEVLEIVMAIFKMITPEDVKLLPDDENTPEKRAEKIWKYFGKNDDDKLTEKEFIEGTLANKEILRLIQFEPQKVKEKMKNA

Approximately 25.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.