RecF/UvrF 蛋白, E.coli (His-SUMO)

RecF/UvrF Protein is crucial in DNA metabolism, essential for DNA replication and inducing the SOS response. Its preference for binding to single-stranded DNA underscores its role in DNA maintenance. RecF also displays an affinity for ATP binding, emphasizing its involvement in regulating DNA-related functions. RecF/UvrF Protein, E.coli (His-SUMO) is the recombinant E. coli-derived RecF/UvrF protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag. The total length of RecF/UvrF Protein, E.coli (His-SUMO) is 356 a.a., with molecular weight of ~56.4 kDa.

RecF/UvrF 蛋白在 DNA 代谢中发挥着至关重要的作用，对于 DNA 复制和 SOS 反应的正确诱导至关重要。它对结合单链、线性 DNA 的偏好凸显了它参与 DNA 维护的复杂过程。此外，RecF 表现出对 ATP 结合的亲和力，进一步强调了其在 DNA 相关功能调节中的作用。

E.coli

E. coli

N-SUMO;N-6*His

P0A7H0 (S2-D357)

948209  [NCBI]

SLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIYTLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDGHKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQRNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTFSFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKLLMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISAEHVIDMSDENSKMFTVEKGKITD

Approximately 56.4 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.