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  3. RecO 蛋白, E.coli (His-SUMO)

RecO 蛋白, E.coli (His-SUMO)

目录号: HY-P71512
COA 产品使用指南

RecO 蛋白是 DNA 修复和 RecF 途径的重要参与者,作为单体发挥作用,有助于 DNA 修复机制的复杂编排并促进重组。它的单体形式强调了它的独特参与,强调了它在协调细胞对 DNA 损伤和重组反应中的重要性。RecO 蛋白, E.coli (His-SUMO) 是重组的 RecO 蛋白,由 E. coli 表达,带有 N-SUMO, N-6*His 标签。RecO 蛋白, E.coli (His-SUMO) 全长 242 个氨基酸,分子量约为 ~43.4 kDa。

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50 μg ¥7500 In-stock
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描述

RecO protein, a crucial participant in DNA repair and the RecF pathway, operates as a monomer, contributing to the intricate choreography of DNA repair mechanisms and facilitating recombination. Its monomeric form underscores its singular involvement, highlighting its significance in orchestrating cellular responses to DNA damage and recombination. RecO Protein, E.coli (His-SUMO) is the recombinant E. coli-derived RecO protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag. The total length of RecO Protein, E.coli (His-SUMO) is 242 a.a., with molecular weight of ~43.4 kDa.

研究背景

RecO 蛋白是 DNA 修复和 RecF 重组途径的重要参与者,作为单体发挥其功能作用。在复杂的细胞过程中,RecO 作为关键角色出现,有助于 DNA 修复机制的复杂编排并促进 RecF 途径内的重组。它的单体形式强调了它的独特参与,突出了它在协调细胞对 DNA 损伤和重组事件的反应中的重要性。

种属

E.coli

表达系统

E. coli

标签

N-SUMO;N-6*His

蛋白编号

P0A7H3 (M1-E242)

基因 ID

947038  [NCBI]

蛋白结构
N-term
6*His-SUMO
RecO (M1-E242)
Accession # P0A7H3
C-term
同用名
recO; b2565; JW2549; DNA repair protein RecO; Recombination protein O
氨基酸序列

MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRSTLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEYETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDDTMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLGGKPLKSRELFRQFMPKRTVKTHYE

分子量

Approximately 43.4 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized after extensive dialysis against solution in 20 mM Tris-HC1, 0.5 M NaCl, 6% Trehalose, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

RecO Protein, E.coli (His-SUMO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
RecO Protein, E.coli (His-SUMO)
目录号:
HY-P71512
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