RelA/NFkB p65 蛋白, Human (GST)

RelA/NF-kappa-B p65 is a key transcription factor that mediates inflammatory, immune, and cellular processes in response to multiple stimuli. Inactive in the cytoplasm, it forms a complex with I-kappa-B. RelA/NFkB p65 Protein, Human (GST) is the recombinant human-derived RelA/NFkB p65, expressed by E. coli , with N-GST labeled tag.

RelA/NF-kappaB p65 是多种细胞过程中不可或缺的多方面转录因子，充当控制炎症、免疫、分化、生长、肿瘤发生和细胞凋亡的信号转导级联的终点。RelA 在 NF-kappaB 异二聚体复合物中发挥作用，主要与 NFKB1/p50 和 REL 配对，发挥靶基因的转录激活作用。该复合物通过与 NF-kappaB 抑制剂 (I-kappa-B) 蛋白结合而在细胞质中保持非活性。受到刺激后，I-kappa-B 被 I-kappa-B 激酶磷酸化，引发其降解，释放活性 NF-kappaB 复合物。RelA 表现出较弱的 DNA 结合位点，直接参与 NF-kappaB 复合物内的 DNA 结合。除了直接转录激活之外，RelA 还调节启动子可及性，间接影响基因表达。有趣的是，RelA 还与多种蛋白质相互作用，包括各种抑制剂、激酶、脱乙酰酶和其他辅助因子，强调了它在复杂的细胞网络中的作用。此外，在微生物感染的背景下，RelA 在 SARS-CoV-2 感染期间调节 IFN 反应中发挥着关键作用，并与潜在的 HIV-1 转录再激活有关。

Human

E. coli

N-GST

Q04206-1 (M1-Y306)

5970  [NCBI]

MDELFPLIFPAEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAGSIPGERSTDTTKTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGFYEAELCPDRCIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFQVPIEEQRGDYDLNAVRLCFQVTVRDPSGRPLRLPPVLSHPIFDNRAPNTAELKICRVNRNSGSCLGGDEIFLLCDKVQKEDIEVYFTGPGWEARGSFSQADVHRQVAIVFRTPPYADPSLQAPVRVSMQLRRPSDRELSEPMEFQYLPDTDDRHRIEEKRKRTY

Approximately 60 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized a 0.2 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.