RIOK1 蛋白, Human (sf9, His-GST)

The RIOK1 protein is critical in the final cytoplasmic maturation of the 40S ribosomal subunit, actively processing 18S-E pre-rRNA into mature 18S rRNA. RIOK1 is critical for NOB1 and PNO1 recycling of 40S late precursors, and its association with 40S late subunits suggests involvement in a translation-like checkpoint cycle. RIOK1 Protein, Human (sf9, His-GST) is the recombinant human-derived RIOK1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of RIOK1 Protein, Human (sf9, His-GST) is 568 a.a., with molecular weight of ~94 kDa.

RIOK1 蛋白在 40S 核糖体亚基细胞质成熟的最后阶段发挥着至关重要的作用，积极参与 18S-E 前 rRNA 到成熟 18S rRNA 的加工。对于从 40S 晚期前体回收 NOB1 和 PNO1 至关重要，RIOK1 与 40S 晚期亚基中间体的关联表明，RIOK1 在与 60S 核糖体亚基结合之前参与了类似翻译的检查点循环。尽管其提议的蛋白激酶结构域主要充当 ATP 酶，但催化活性动态调节其与 40S 亚基的关联。除了在核糖体生物发生中的作用之外，RIOK1 还充当衔接蛋白，促进 NCL/核仁素募集到 PRMT5 复合物中以实现对称甲基化，证明了其对细胞过程的多方面贡献。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

N-His;N-GST

Q9BRS2 (M1-K568)

83732  [NCBI]

Approximately 94 kDa

Greater than 85% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.