RIPK2 蛋白, Human (P. pastoris, His)

RIPK2 is a multifunctional serine/threonine/tyrosine protein kinase that coordinates innate and adaptive immune responses. It is activated by bacterial peptidoglycan to form filaments in the NOD1 and NOD2 pathways, undergoing autophosphorylation and polyubiquitination. RIPK2 Protein, Human (P. pastoris, His) is the recombinant human-derived RIPK2 protein, expressed by P. pastoris , with C-6*His, C-6*His labeled tag. The total length of RIPK2 Protein, Human (P. pastoris, His) is 540 a.a., with molecular weight of 66 kDa.

RIPK2 是一种丝氨酸/苏氨酸/酪氨酸蛋白激酶，在协调先天性和适应性免疫反应中发挥着关键作用。作为 NOD1 和 NOD2 信号通路中的关键效应器，RIPK2 在被细菌肽聚糖激活后形成细丝，并通过 CARD-CARD 结构域招募。随后发生自磷酸化和多泛素化，涉及 XIAP、BIRC2 和 BIRC3 的“Lys-63”连接泛素链，以及 LUBAC 复合物的“Met-1”连接多泛素化。这会将 RIPK2 转化为支架蛋白，招募下游效应子。RIPK2 上“Lys-63”连接的多聚泛素链促进 IKBKG/NEMO 的募集，启动 IKBKB/IKKB 的激活以及随后抑制剂中 NF-kappa-B 的释放。虽然激酶活性对于 NOD1 和 NOD2 途径来说是可有可无的，但 RIPK2 有助于 ARHGEF2 的酪氨酸磷酸化，通过 NOD2 激活 NF-kappa-B。此外，在适应性免疫中，RIPK2 在 T 细胞受体参与过程中促进 BCL10 磷酸化，并参与响应 NGFR 信号传导的 RHOA 失活。这种多方面的激酶是多种免疫过程中的关键介质。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

P. pastoris

C-6*His

O43353 (M1-M540)

8767  [NCBI]

MNGEAICSALPTIPYHKLADLRYLSRGASGTVSSARHADWRVQVAVKHLHIHTPLLDSERKDVLREAEILHKARFSYILPILGICNEPEFLGIVTEYMPNGSLNELLHRKTEYPDVAWPLRFRILHEIALGVNYLHNMTPPLLHHDLKTQNILLDNEFHVKIADFGLSKWRMMSLSQSRSSKSAPEGGTIIYMPPENYEPGQKSRASIKHDIYSYAVITWEVLSRKQPFEDVTNPLQIMYSVSQGHRPVINEESLPYDIPHRARMISLIESGWAQNPDERPSFLKCLIELEPVLRTFEEITFLEAVIQLKKTKLQSVSSAIHLCDKKKMELSLNIPVNHGPQEESCGSSQLHENSGSPETSRSLPAPQDNDFLSRKAQDCYFMKLHHCPGNHSWDSTISGSQRAAFCDHKTTPCSSAIINPLSTAGNSERLQPGIAQQWIQSKREDIVNQMTEACLNQSLDALLSRDLIMKEDYELVSTKPTRTSKVRQLLDTTDIQGEEFAKVIVQKLKDNKQMGLQPYPEILVVSRSPSLNLLQNKSM

Approximately 66 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.