1. 重组蛋白
  2. Enzymes & Regulators Ubiquitin Related Proteins
  3. Transferases (EC 2) Ubiquitin Enzymes
  4. E3 Ligases
  5. RNF181 蛋白, Human

RNF181 蛋白, Human

目录号: HY-P701560
产品使用指南

RNF181 蛋白是一种 E3 泛素蛋白连接酶,介导 26S 蛋白酶体亚基 PSMC2/RPT1 的单泛素化。这表明 26S 蛋白酶体具有调节作用,26S 蛋白酶体是蛋白质降解的重要细胞机制。RNF181 调节 PSMC2/RPT1 的能力意味着参与控制细胞蛋白质稳态的复杂调节网络。RNF181 蛋白, Human 是重组的 RNF181 蛋白,由 E. coli 表达,不带标签。RNF181 蛋白, Human 全长 152 个氨基酸。

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描述

RNF181 Protein, an E3 ubiquitin-protein ligase, mediates the monoubiquitination of the 26S proteasome subunit PSMC2/RPT1. This suggests a role in regulating the 26S proteasome, a vital cellular machinery for protein degradation. RNF181's capacity to modulate PSMC2/RPT1 implies involvement in the intricate regulatory network governing cellular protein homeostasis. RNF181 Protein, Human is the recombinant human-derived RNF181 protein, expressed by E. coli , with tag free. The total length of RNF181 Protein, Human is 152 a.a., .

研究背景

RNF181 蛋白作为 E3 泛素蛋白连接酶,通过从 E2 泛素结合酶以硫酯的形式接受泛素,然后直接将泛素转移至其目标底物来发挥作用。值得注意的是,RNF181 催化 26S 蛋白酶体亚基 PSMC2/RPT1 的单泛素化。这种活性表明其在 26S 蛋白酶体的调节中发挥作用,26S 蛋白酶体是负责蛋白质降解的重要细胞机制。RNF181 介导 PSMC2/RPT1 单泛素化的能力意味着可能参与蛋白酶体功能的调节,有助于控制细胞蛋白质稳态的复杂调节网络。

种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

Q9P0P0 (A2-T153)

基因 ID

51255

蛋白结构
N-term
RNF181 (A2-T153)
Accession # Q9P0P0
C-term
同用名
RNF181; E3 ubiquitin-protein ligase RNF181; RING finger protein 181
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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