1. 重组蛋白
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. Rnls 蛋白, Mouse (His-SUMO)

Rnls 蛋白, Mouse (His-SUMO)

目录号: HY-P71521
产品使用指南

Rnls 蛋白催化丰度较低的 1,2-二氢-β-NAD(P) 和 1,6-二氢-β-NAD(P) 氧化形成 β-NAD(P)(+)。这种酶主要由肾脏分泌,在血液中循环,在调节心脏功能和全身血压方面发挥着至关重要的作用。Rnls 蛋白, Mouse (His-SUMO) 是重组的 Rnls 蛋白,由 E. coli 表达,带有 N-His, N-SUMO 标签。

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描述

The Rnls protein catalyzes the oxidation of the less abundant 1,2-dihydro-β-NAD(P) and 1,6-dihydro-β-NAD(P) to form β-NAD(P)(+). Mainly secreted by the kidneys, this enzyme circulates in the blood and plays a vital role in regulating heart function and systemic blood pressure. Rnls Protein, Mouse (His-SUMO) is the recombinant mouse-derived Rnls protein, expressed by E. coli , with N-His, N-SUMO labeled tag.

研究背景

肾酶 (Rnls) 是一种催化含量较低的 1,2-二氢-β-NAD(P) 和 1,6-二氢-β-NAD(P) 氧化形成 β-NAD(P)(+ )。这种酶主要由肾脏分泌,在血液中循环,在调节心脏功能和全身血压方面发挥着至关重要的作用。肾酶已被确定为降低体内血压的关键因素,通过降低心肌收缩力和心率,同时防止外周血管张力代偿性增加来实现。这表明肾酶、血浆儿茶酚胺水平升高和心血管风险升高之间存在因果关系。值得注意的是,高浓度的儿茶酚胺会激活血浆肾酶,促进其分泌和合成。肾酶的多方面功能强调了其在心血管调节中的重要性及其作为控制血压和心血管健康的治疗靶点的潜力。

种属

Mouse

表达系统

E. coli

标签

N-His;N-SUMO

蛋白编号

A7RDN6-1 (A18-I342)

基因 ID
蛋白结构
N-term
6*His-SUMO
Rnls (A18-I342)
Accession # A7RDN6-1
C-term
同用名
Rnls; Renalase; EC 1.6.3.5; Monoamine oxidase-C; MAO-C; mMAO-C
氨基酸序列

ALLRKEITAPLYLGLWDKGGDIGGRMITASSPHNPRCTADLGAQYITCSPHYVKEHQNFYEELLAHGILKPLTSPIEGMKGKEGDCNFVAPQGFSSVIKYYLKKSGAEVSLKHCVTQIHLKDNKWEVSTDTGSAEQFDLVILTMPAPQILELQGDIVNLISERQREQLKSVSYSSRYALGLFYEVGMKIGVPWSCRYLSSHPCICFISIDNKKRNIESSECGPSVVIQTTVPFGVQHLEASEADVQKLMIQQLETILPGLPQPVATICHKWTYSQVTSSVSDRPGQMTLHLKPFLVCGGDGFTHSNFNGCISSALSVMKVLKRYI

分子量

Approximately 52.0 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Rnls Protein, Mouse (His-SUMO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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