RPRD1B 蛋白, Human (HEK293, N-His)

RPRD1B, is a necessary scaffolding protein that maintains genetic integrity by regulating resolution of R-loops at both the transcription termination and DNA double-strand break (DSB) repair levels. In endometrial cancers, RPRD1B accelerates cell cycle through up-regulating Cyclin D1, CDK4, and CDK6. In addition, RPRD1B enhancse transcription of CCND1 and promotes cell proliferation by interacting with RNA polymerase II. RPRD1B enhances the β-Catenin·TCF4 transcriptional activity in response to Wnt signaling^[1][2][3][4]. RPRD1B Protein, Human (HEK293, N-His) is the recombinant human-derived RPRD1B protein, expressed by HEK293 , with N-6*His labeled tag.

RPRD1B，也称为 Ku70 结合蛋白 5-Hera 或 CREPT，是一种必要的支架蛋白，通过在转录终止和 DNA 双链断裂 (DSB) 修复水平上调节 R 环的分辨率来维持遗传完整性 ^[1]。RPRD1B 在多种癌症中表达上调，并调节基因组稳定性和转录终止^[2]。在子宫内膜癌中，RPRD1B 通过上调 Cyclin D1、CDK4 和 CDK6 (细胞周期 G1/S 相变的主要调节因子) 来加速细胞周期^[3]。此外，RPRD1B 通过与 RNA 聚合酶 II 相互作用增强 CCND1 的转录并促进细胞增殖。RPRD1B 增强 β-Catenin·TCF4 转录活性以响应 Wnt 信号传导^[4]。

Human

HEK293

N-6*His

AAH33629.1 (M1-D326)

58490

MSSFSESALEKKLSELSNSQHSVQTLSLWLIHHRKHAGPIVSVWHRELRKAKSNRKLTFLYLANDVIQNSKRKGPEFTREFESVLVDAFSHVAREADEGCKKPLERLLNIWQERSVYGGEFIQQLKLSMEDSKSPPPKATEEKKSLKRTFQQIQEEEDDDYPGSYSPQDPSAGPLLTEELIKALQDLENAASGDATVRQKIASLPQEVQDVSLLEKITDKEAAERLSKTVDEACLLLAEYNGRLAAELEDRRQLARMLVEYTQNQKDVLSEKEKKLEEYKQKLARVTQVRKELKSHIQSLPDLSLLPNVTGGLAPLPSAGDLFSTD

37-42 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.