RUVBL1 蛋白, Human (sf9, His)

The RUVBL1 protein has DNA-stimulated ATPase and DNA helicase activities and can hexamerize for ATP hydrolysis. It is a member of the NuA4 complex and contributes to histone acetylation to activate genes. RUVBL1 Protein, Human (sf9, His) is the recombinant human-derived RUVBL1 protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of RUVBL1 Protein, Human (sf9, His) is 456 a.a., with molecular weight of ~57 kDa.

RUVBL1 蛋白具有单链 DNA 刺激的 ATP 酶和 ATP 依赖性 DNA 解旋酶（3' 至 5'）活性，六聚化被认为对 ATP 水解至关重要，环状结构中的相邻亚基有助于 ATP 酶活性。作为 NuA4 组蛋白乙酰转移酶复合物的组成部分，RUVBL1 主要通过核小体组蛋白 H4 和 H2A 的乙酰化参与选定基因的转录激活。这种修饰可以改变核小体-DNA 相互作用，并促进与其他正向调节转录的蛋白质的相互作用。NuA4 复合物（包括 ATP 酶和解旋酶活性）部分是由 RUVBL1 和 RUVBL2 与 EP400 的结合所促成。当 NuA4 复合物被招募到 DNA 损伤位点时，也可能在 DNA 修复中发挥直接作用。此外，RUVBL1 是 SWR1 样复合物的一个组成部分，负责从核小体去除组蛋白 H2A.Z/H2AZ1，并被提议作为染色质重塑 INO80 复合物的核心成分，该复合物表现出 DNA 和核小体激活的 ATP 酶活性并催化 ATP 依赖性核小体滑动。RUVBL1 对于细胞增殖至关重要，在 MYC 的致癌转化中发挥作用，并通过 LEF1/TCF1-CTNNB1 复合物调节转录激活。此外，RUVBL1 可以结合细胞表面的纤溶酶原，增强纤溶酶原的激活。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

N-His

Q9Y265 (M1-K456)

8607  [NCBI]

Approximately 57 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 10% Glycerol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.