S100A1 Protein, Human (His)

S100A1 is a small calcium-binding protein that plays critical roles in multiple processes, including Ca(2+) homeostasis and cardiomyocyte regulation. Elevated intracellular Ca(2+) triggers conformational changes in S100A1, thereby interacting with key proteins in cardiomyocytes such as RYR1, RYR2, ATP2A2 and F1-ATPase. S100A1 Protein, Human (His) is the recombinant human-derived S100A1 protein, expressed by E. coli , with N-His labeled tag. The total length of S100A1 Protein, Human (His) is 94 a.a., with molecular weight of ~11.5 kDa.

S100A1 是一种小型钙结合蛋白，在各种生物过程中发挥着至关重要的作用，包括 Ca(2+) 稳态、软骨细胞生物学和心肌细胞调节。当细胞内 Ca(2+) 水平增加时，S100A1 与钙结合，引发构象变化，促进与特定靶蛋白的相互作用，随后调节其活性。特别是，在心肌细胞中，S100A1 通过与兰尼碱受体 RYR1 和 RYR2、肌浆网 Ca(2+)-ATPase/ATP2A2 和线粒体 F1- 等关键蛋白相互作用，协调控制肌浆网 Ca(2+) 循环和线粒体功能的网络。ATP酶。这种多面蛋白质还有助于舒张期 Ca(2+) 解离和肌丝力学，增强舒张期的松弛。S100A1 以两条 α 链、两条 β 链或各一条的二聚体形式存在，并与 S100P 形成异二聚体。此外，它还与 AGER、CAPZA1、FKBP4、RYR1、RYR2、CACYBP、PPP5C、ATP2A2、PLN、ATP5F1A 和 ATP5F1B 等蛋白质进行各种相互作用，以钙依赖性方式参与多种细胞过程。

Human

E. coli

N-His

P23297 (M1-S94)

6271  [NCBI]

MGSELETAMETLINVFHAHSGKEGDKYKLSKKELKELLQTELSGFLDAQKDVDAVDKVMKELDENGDGEVDFQEYVVLVAALTVACNNFFWENS

Approximately 11.5 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 0.1 mM EDTA, pH 7.0.

<1 EU/μg; determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.